Refine
Year of publication
- 2024 (9)
- 2023 (23)
- 2022 (32)
- 2021 (38)
- 2020 (40)
- 2019 (52)
- 2018 (46)
- 2017 (45)
- 2016 (32)
- 2015 (43)
- 2014 (42)
- 2013 (49)
- 2012 (41)
- 2011 (69)
- 2010 (61)
- 2009 (76)
- 2008 (51)
- 2007 (48)
- 2006 (32)
- 2005 (40)
- 2004 (75)
- 2003 (39)
- 2002 (45)
- 2001 (49)
- 2000 (53)
- 1999 (31)
- 1998 (32)
- 1997 (29)
- 1996 (27)
- 1995 (18)
- 1994 (9)
- 1993 (18)
- 1992 (11)
- 1991 (11)
- 1990 (15)
- 1989 (17)
- 1988 (20)
- 1987 (19)
- 1986 (6)
- 1985 (8)
- 1984 (7)
- 1983 (5)
- 1982 (20)
- 1981 (15)
- 1980 (29)
- 1979 (20)
- 1978 (25)
- 1977 (13)
- 1976 (16)
- 1975 (12)
- 1974 (4)
- 1973 (2)
- 1972 (6)
- 1968 (2)
- 1967 (1)
Document Type
- Article (1578) (remove)
Keywords
- Einspielen <Werkstoff> (7)
- FEM (4)
- Finite-Elemente-Methode (4)
- LAPS (4)
- CellDrum (3)
- Label-free detection (3)
- biosensors (3)
- hydrogen peroxide (3)
- shakedown analysis (3)
- Bacillus atrophaeus (2)
- Bauingenieurwesen (2)
- CAD (2)
- Capacitive field-effect sensor (2)
- Einspielanalyse (2)
- Empirical process (2)
- Field-effect sensor (2)
- Goodness-of-fit test (2)
- Independence test (2)
- Light-addressable potentiometric sensor (2)
- Lipopolysaccharide (2)
Institute
- Fachbereich Medizintechnik und Technomathematik (1578) (remove)
To gain insight on chemical sterilization processes, the influence of temperature (up to 70 °C), intense green light, and hydrogen peroxide (H₂O₂) concentration (up to 30% in aqueous solution) on microbial spore inactivation is evaluated by in-situ Raman spectroscopy with an optical trap. Bacillus atrophaeus is utilized as a model organism. Individual spores are isolated and their chemical makeup is monitored under dynamically changing conditions (temperature, light, and H₂O₂ concentration) to mimic industrially relevant process parameters for sterilization in the field of aseptic food processing. While isolated spores in water are highly stable, even at elevated temperatures of 70 °C, exposure to H₂O₂ leads to a loss of spore integrity characterized by the release of the key spore biomarker dipicolinic acid (DPA) in a concentration-dependent manner, which indicates damage to the inner membrane of the spore. Intensive light or heat, both of which accelerate the decomposition of H₂O₂ into reactive oxygen species (ROS), drastically shorten the spore lifetime, suggesting the formation of ROS as a rate-limiting step during sterilization. It is concluded that Raman spectroscopy can deliver mechanistic insight into the mode of action of H₂O₂-based sterilization and reveal the individual contributions of different sterilization methods acting in tandem.