Refine
Year of publication
Institute
- Fachbereich Medizintechnik und Technomathematik (2077) (remove)
Document Type
- Article (1594)
- Conference Proceeding (243)
- Book (96)
- Part of a Book (62)
- Doctoral Thesis (27)
- Patent (17)
- Report (15)
- Other (9)
- Habilitation (4)
- Lecture (3)
Keywords
- Biosensor (25)
- Finite-Elemente-Methode (16)
- CAD (15)
- civil engineering (14)
- Bauingenieurwesen (13)
- Einspielen <Werkstoff> (13)
- shakedown analysis (9)
- FEM (6)
- Limit analysis (6)
- Shakedown analysis (6)
Label-free electrical detection of consecutive deoxyribonucleic acid (DNA) hybridization/denaturation by means of an array of individually addressable field-effect-based nanoplate silicon-on-insulator (SOI) capacitors modified with gold nanoparticles (Au-NP) is investigated. The proposed device detects charge changes on Au-NP/DNA hybrids induced by the hybridization or denaturation event. DNA hybridization was performed in a high ionic-strength solution to provide a high hybridization efficiency. On the other hand, to reduce the screening of the DNA charge by counter ions and to achieve a high sensitivity, the sensor signal induced by the hybridization and denaturation events was measured in a low ionic-strength solution. High sensor signals of about 120, 90, and 80 mV were registered after the DNA hybridization, denaturation, and re-hybridization events, respectively. Fluorescence microscopy has been applied as reference method to verify the DNA immobilization, hybridization, and denaturation processes. An electrostatic charge-plane model for potential changes at the gate surface of a nanoplate field-effect sensor induced by the DNA hybridization has been developed taking into account both the Debye length and the distance of the DNA charge from the gate surface.