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- 2021 (4) (remove)
Keywords
- Capacitive field-effect sensor (1)
- Label-free detection (1)
- Plant virus (1)
- TMV adsorption (1)
- Ta₂O₅ gate (1)
- Tobacco mosaic virus (TMV) (1)
- Zeta potential (1)
- acetoin (1)
- acetoin reductase (1)
- actuator-sensor system (1)
- alcoholic beverages (1)
- biosensors (1)
- capacitive field-effect sensor (1)
- capacitive field-effect sensors (1)
- enzyme kinetics (1)
- light-addressable electrode (1)
- light-addressable potentiometric sensor (1)
- microfluidics (1)
- plant virus detection (1)
- tobacco mosaic virus (TMV) (1)
An acetoin biosensor based on a capacitive electrolyte–insulator–semiconductor (EIS) structure modified with the enzyme acetoin reductase, also known as butane-2,3-diol dehydrogenase (Bacillus clausii DSM 8716ᵀ), is applied for acetoin detection in beer, red wine, and fermentation broth samples for the first time. The EIS sensor consists of an Al/p-Si/SiO₂/Ta₂O₅ layer structure with immobilized acetoin reductase on top of the Ta₂O₅ transducer layer by means of crosslinking via glutaraldehyde. The unmodified and enzyme-modified sensors are electrochemically characterized by means of leakage current, capacitance–voltage, and constant capacitance methods, respectively.