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Keywords
- Acyl-amino acids (2)
- Aminoacylase (2)
- Acylation (1)
- Biocatalysis (1)
- Biosurfactants (1)
- Chaperone (1)
- Chaperone co-expression (1)
- Inclusion bodies (1)
- Streptomyces griseus (1)
- Streptomyces lividans (1)
- Vibrio natriegens (1)
- recombinant expression (1)
- α-aminoacylase (1)
- ε-lysine acylase (1)
An improved and convenient ninhydrin assay for aminoacylase activity measurements was developed using the commercial EZ Nin™ reagent. Alternative reagents from literature were also evaluated and compared. The addition of DMSO to the reagent enhanced the solubility of Ruhemann's purple (RP). Furthermore, we found that the use of a basic, aqueous buffer enhances stability of RP. An acidic protocol for the quantification of lysine was developed by addition of glacial acetic acid. The assay allows for parallel processing in a 96-well format with measurements microtiter plates.