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Author

  • Thomas Bronder (18)
  • Michael Josef Schöning (17)
  • Arshak Poghossian (16)
  • Chunsheng Wu (11)
  • Michael Keusgen (5)
  • Frederik Werner (4)
  • M. Keusgen (3)
  • Christina Wege (2)
  • Claudia Koch (2)
  • Matthias Bäcker (2)
  • Max P. Jessing (2)
  • Melanie Jablonski (2)
  • S. Scheja (2)
  • Sabrina Scheja (2)
  • Alexander Goretzki (1)
  • C. Metzger-Boddien (1)
  • Christoph Metzger-Boddien (1)
  • David Rolka (1)
  • Dieter Mewes (1)
  • Frank Gerlach (1)
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Year of publication

  • 2020 (1)
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  • 2017 (3)
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Document Type

  • Article (12)
  • Conference Proceeding (5)
  • Doctoral Thesis (1)

Keywords

  • LAPS (2)
  • DNA biosensor (1)
  • DNA hybridization (1)
  • Field effect (1)
  • Label-free detection (1)
  • Layer-by-layer adsorption (1)
  • Poly(allylamine hydrochloride) (1)
  • field-effect sensor (1)
  • lable-free detection (1)

Institute

  • Fachbereich Medizintechnik und Technomathematik (18)
  • INB - Institut für Nano- und Biotechnologien (17)

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Field-Effect Biosensors Modified with Tobacco Mosaic Virus Nanotubes as Enzyme Nanocarrier (2017)
Melanie Jablonski ; Claudia Koch ; Thomas Bronder ; Arshak Poghossian ; Christina Wege ; Michael Josef Schöning
Field-effect biosensor using virus particles as scaffolds for enzyme immobilization (2018)
Arshak Poghossian ; Melanie Jablonski ; Claudia Koch ; Thomas Bronder ; David Rolka ; Christina Wege ; Michael Josef Schöning
A field-effect biosensor employing tobacco mosaic virus (TMV) particles as scaffolds for enzyme immobilization is presented. Nanotubular TMV scaffolds allow a dense immobilization of precisely positioned enzymes with retained activity. To demonstrate feasibility of this new strategy, a penicillin sensor has been developed by coupling a penicillinase with virus particles as a model system. The developed field-effect penicillin biosensor consists of an Al-p-Si-SiO₂-Ta₂O₅-TMV structure and has been electrochemically characterized in buffer solutions containing different concentrations of penicillin G. In addition, the morphology of the biosensor surface with virus particles was characterized by scanning electron microscopy and atomic force microscopy methods. The sensors possessed a high penicillin sensitivity of ~ 92 mV/dec in a nearly-linear range from 0.1 mM to 10 mM, and a low detection limit of about 50 µM. The long-term stability of the penicillin biosensor was periodically tested over a time period of about one year without any significant loss of sensitivity. The biosensor has also been successfully applied for penicillin detection in bovine milk samples.
Detection of PCR-Amplified Tuberculosis DNA Fragments with Polyelectrolyte-Modified Field-Effect Sensors (2018)
Thomas Bronder ; Max P. Jessing ; Arshak Poghossian ; Michael Keusgen ; Michael Josef Schöning
Field-effect-based electrolyte-insulator-semiconductor (EIS) sensors were modified with a bilayer of positively charged weak polyelectrolyte (poly(allylamine hydrochloride) (PAH)) and probe single-stranded DNA (ssDNA) and are used for the detection of complementary single-stranded target DNA (cDNA) in different test solutions. The sensing mechanism is based on the detection of the intrinsic molecular charge of target cDNA molecules after the hybridization event between cDNA and immobilized probe ssDNA. The test solutions contain synthetic cDNA oligonucleotides (with a sequence of tuberculosis mycobacteria genome) or PCR-amplified DNA (which origins from a template DNA strand that has been extracted from Mycobacterium avium paratuberculosis-spiked human sputum samples), respectively. Sensor responses up to 41 mV have been measured for the test solutions with DNA, while only small signals of ∼5 mV were detected for solutions without DNA. The lower detection limit of the EIS sensors was ∼0.3 nM, and the sensitivity was ∼7.2 mV/decade. Fluorescence experiments using SybrGreen I fluorescence dye support the electrochemical results.
Development of a Combined pH- and Redox-Sensitive Bi-Electrode Glass Thin-Film Sensor (2019)
Heiko Iken ; Thomas Bronder ; Alexander Goretzki ; Jana Kriesel ; Kristina Ahlborn ; Frank Gerlach ; Winfried Vonau ; Willi Zander ; Jürgen Schubert ; Michael Josef Schöning
Label-free detection of double-stranded DNA molecules with polyelectrolyte-modified capacitive field-effect sensors (2017)
Thomas Bronder ; Arshak Poghossian ; Michael Keusgen ; Michael Josef Schöning
Label-Free DNA Detection with Capacitive Field-Effect Devices—Challenges and Opportunities (2017)
Michael Josef Schöning ; Thomas Bronder ; Chunsheng Wu ; Sabrina Scheja ; Max Jessing ; Christoph Metzger-Boddien ; Michael Keusgen ; Arshak Poghossian
Field-effect EIS (electrolyte-insulator-semiconductor) sensors modified with a positively charged weak polyelectrolyte layer have been applied for the electrical detection of DNA (deoxyribonucleic acid) immobilization and hybridization by the intrinsic molecular charge. The EIS sensors are able to detect the existence of target DNA amplicons in PCR (polymerase chain reaction) samples and thus, can be used as tool for a quick verification of DNA amplification and the successful PCR process. Due to their miniaturized setup, compatibility with advanced micro- and nanotechnologies, and ability to detect biomolecules by their intrinsic molecular charge, those sensors can serve as possible platform for the development of label-free DNA chips. Possible application fields as well as challenges and limitations will be discussed.
DNA Immobilization and Hybridization Detection by the Intrinsic Molecular Charge Using Capacitive Field-Effect Sensors Modified with a Charged Weak Polyelectrolyte Layer (2015)
Thomas Bronder ; Arshak Poghossian ; Sabrina Scheja ; Chunsheng Wu ; Michael Keusgen ; Dieter Mewes ; Michael Josef Schöning
An application of a scanning light-addressable potentiometric sensor for label-free DNA detection (2013)
Chunsheng Wu ; Arshak Poghossian ; Frederik Werner ; Thomas Bronder ; Matthias Bäcker ; Ping Wang ; Michael Josef Schöning
Label-free detection of DNA hybridization with light-addressable potentiometric sensors: comparison of various DNA-immobilization strategies (2014)
Thomas Bronder ; Chunsheng Wu ; Arshak Poghossian ; Frederik Werner ; M. Keusgen ; Michael Josef Schöning
Light-addressable potentiometric sensors (LAPS) consisting of a p-Si-SiO2 and p-Si-SiO2-Au structure, respectively, have been tested for a label-free electrical detection of DNA (deoxyribonucleic acid) hybridization. Three different strategies for immobilizing single-stranded probe DNA (ssDNA) molecules on a LAPS surface have been studied and compared: (a) immobilization of thiol-modified ssDNA on the patterned Au surface via gold-thiol bond, (b) covalent immobilization of amino-modified ssDNA onto the SiO2 surface functionalized with 3-aminopropyltriethoxysilane and (c) layer-by-layer adsorption of negatively charged ssDNA on a positively charged weak polyelectrolyte layer of poly(allylamine hydrochloride).
Label-free electrical detection of DNA with a multi-spot LAPS: First step towards light-addressable DNA chips (2014)
Chunsheng Wu ; Thomas Bronder ; Arshak Poghossian ; Frederik Werner ; Matthias Bäcker ; Michael Josef Schöning
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