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Label-free detection of DNA hybridization with light-addressable potentiometric sensors: comparison of various DNA-immobilization strategies (2014)
Bronder, Thomas ; Wu, Chunsheng ; Poghossian, Arshak ; Werner, Frederik ; Keusgen, M. ; Schöning, Michael Josef
Light-addressable potentiometric sensors (LAPS) consisting of a p-Si-SiO2 and p-Si-SiO2-Au structure, respectively, have been tested for a label-free electrical detection of DNA (deoxyribonucleic acid) hybridization. Three different strategies for immobilizing single-stranded probe DNA (ssDNA) molecules on a LAPS surface have been studied and compared: (a) immobilization of thiol-modified ssDNA on the patterned Au surface via gold-thiol bond, (b) covalent immobilization of amino-modified ssDNA onto the SiO2 surface functionalized with 3-aminopropyltriethoxysilane and (c) layer-by-layer adsorption of negatively charged ssDNA on a positively charged weak polyelectrolyte layer of poly(allylamine hydrochloride).
Electrostatic Detection of Unlabelled Single- and Double-stranded DNA Using Capacitive Field-effect Devices Functionalized with a Positively Charged Polyelectrolyte Layer (2015)
Bronder, Thomas ; Poghossian, Arshak ; Scheja, S. ; Wu, Chunsheng ; Keusgen, M. ; Schöning, Michael Josef
Capacitive field-effect electrolyte-insulator-semiconductor sensors consisting of an Al-p-Si-SiO2 structure have been used for the electrical detection of unlabelled single- and double-stranded DNA (dsDNA) molecules by their intrinsic charge. A simple functionalization protocol based on the layer-by-layer (LbL) technique was used to prepare a weak polyelectrolyte/probe-DNA bilayer, followed by the hybridization with complementary target DNA molecules. Due to the flat orientation of the LbL-adsorbed DNA molecules, a high sensor signal has been achieved. In addition, direct label-free detection of in-solution hybridized dsDNA molecules has been studied.
An application of a scanning light-addressable potentiometric sensor for label-free DNA detection (2013)
Wu, Chunsheng ; Poghossian, Arshak ; Werner, Frederik ; Bronder, Thomas ; Bäcker, Matthias ; Wang, Ping ; Schöning, Michael Josef
DNA-hybridization detection using light-addressable potentiometric sensor modified with gold layer (2014)
Wu, Chunsheng ; Bronder, Thomas ; Poghossian, Arshak ; Schöning, Michael Josef
Handheld measurement device for field-effect sensor structures: Practical evaluation and limitations (2007)
Wagner, Torsten ; Maris, Rob J. ; Ackermann, Hans-Josef ; Otto, Ralph ; Beging, Stefan ; Poghossian, Arshak ; Schöning, Michael Josef
Feldeffektbasierender Ca2+-sensitiver Sensor für den Einsatz im Nativurin zur Bestimmung des Harnsteinbildungsrisikos (2008)
Beging, Stefan ; Poghossian, Arshak ; Schöning, Michael Josef ; Hataihimakul, Sudkanung ; Busch, Heinz ; Baldsiefen, Gerhard ; Laube, N. ; Kleinen, Lisa ; Hosseiny, Reyhaneh
Online-Messsysteme für die automatisierte Charakterisierung von feldeffektbasierten Biosensoren (2007)
Wagner, Torsten ; Beging, Stefan ; Rotter, L. ; Poghossian, Arshak ; Biselli, Manfred ; Zang, Werner ; Schöning, Michael Josef
Detection of Adrenaline Based on Bioelectrocatalytical System to Support Tumor Diagnostic Technology (2017)
Molinnus, Denise ; Hardt, Gabriel ; Käver, Larissa ; Willenberg, Holger S. ; Poghossian, Arshak ; Keusgen, Michael ; Schöning, Michael Josef
An amperometric biosensor based on the bioelectrocatalytic measurement principle for the detection of adrenaline has been developed. The adrenaline sensor has been prepared by modification of a platinum thin-film electrode with a pyrroloquinoline quinone-dependent glucose dehydrogenase. The enzyme was immobilized via cross-linking method. Lower detection limit of 1 nM of adrenaline has been achieved by measuring at physiological level at pH 7.4.
K+-selective field-effect sensors as transducers for bioelectronic applications (2003)
Mourzina, Yulia ; Mai, T. ; Poghossian, Arshak ; Ermolenko, Yuri E. ; Yoshinobu, Tatsuo ; Vlasov, Yu. G. G. ; Iwasaki, Hiroshi ; Schöning, Michael Josef
Impedance effect of an ion-sensitive membrane: characterisation of an EMIS sensor by impedance spectroscopy, capacitance-voltage and constant-capacitance method (2004)
Poghossian, Arshak ; Mai, D.-T. ; Mourzina, Y. ; Schöning, Michael Josef
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