Refine
Institute
- Fachbereich Medizintechnik und Technomathematik (5) (remove)
Language
- English (5)
Document Type
- Article (5)
Keywords
- acetoin (1)
- acetoin reductase (1)
- alcoholic beverages (1)
- biosensors (1)
- capacitive field-effect sensors (1)
Is part of the Bibliography
- no (5)
An acetoin biosensor based on a capacitive electrolyte–insulator–semiconductor (EIS) structure modified with the enzyme acetoin reductase, also known as butane-2,3-diol dehydrogenase (Bacillus clausii DSM 8716ᵀ), is applied for acetoin detection in beer, red wine, and fermentation broth samples for the first time. The EIS sensor consists of an Al/p-Si/SiO₂/Ta₂O₅ layer structure with immobilized acetoin reductase on top of the Ta₂O₅ transducer layer by means of crosslinking via glutaraldehyde. The unmodified and enzyme-modified sensors are electrochemically characterized by means of leakage current, capacitance–voltage, and constant capacitance methods, respectively.