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  • Schöning, Michael Josef (110)
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  • light-addressable potentiometric sensor (2)
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Electrochemical Evaluation of Light‐Addressable Electrodes Based on TiO2 for the Integration in Lab‐on‐Chip Systems (2018)
Welden, Rene ; Scheja, Sabrina ; Schöning, Michael Josef ; Wagner, Patrick ; Wagner, Torsten
In lab-on-chip systems, electrodes are important for the manipulation (e.g., cell stimulation, electrolysis) within such systems. An alternative to commonly used electrode structures can be a light-addressable electrode. Here, due to the photoelectric effect, the conducting area can be adjusted by modification of the illumination area which enables a flexible control of the electrode. In this work, titanium dioxide based light-addressable electrodes are fabricated by a sol–gel technique and a spin-coating process, to deposit a thin film on a fluorine-doped tin oxide glass. To characterize the fabricated electrodes, the thickness, and morphological structure are measured by a profilometer and a scanning electron microscope. For the electrochemical behavior, the dark current and the photocurrent are determined for various film thicknesses. For the spatial resolution behavior, the dependency of the photocurrent while changing the area of the illuminated area is studied. Furthermore, the addressing of single fluid compartments in a three-chamber system, which is added to the electrode, is demonstrated.
pH sensitivity of nanocrystalline diamond films (2007)
Christiaens, Pieter ; Abouzar, Maryam H. ; Poghossian, Arshak ; Wagner, Torsten ; Bijnens, Nathalie ; Williams, Oliver A. ; Daenen, Michael ; Haenen, Ken ; Douthéret, Olivier ; D'Haen, Jan ; Mekhalif, Z. ; Schöning, Michael Josef ; Wagner, Patrick
Quantitative differential monitoring of the metabolic activity of Corynebacterium glutamicum cultures utilizing a light-addressable potentiometric sensor system (2019)
Dantism, Shahriar ; Röhlen, Desiree ; Selmer, Thorsten ; Wagner, Torsten ; Wagner, Patrick ; Schöning, Michael Josef
Light-Addressable Electrodes for Dynamic and Flexible Addressing of Biological Systems and Electrochemical Reactions (2020)
Welden, Rene ; Schöning, Michael Josef ; Wagner, Patrick H. ; Wagner, Torsten
In this review article, we are going to present an overview on possible applications of light-addressable electrodes (LAE) as actuator/manipulation devices besides classical electrode structures. For LAEs, the electrode material consists of a semiconductor. Illumination with a light source with the appropiate wavelength leads to the generation of electron-hole pairs which can be utilized for further photoelectrochemical reaction. Due to recent progress in light-projection technologies, highly dynamic and flexible illumination patterns can be generated, opening new possibilities for light-addressable electrodes. A short introduction on semiconductor–electrolyte interfaces with light stimulation is given together with electrode-design approaches. Towards applications, the stimulation of cells with different electrode materials and fabrication designs is explained, followed by analyte-manipulation strategies and spatially resolved photoelectrochemical deposition of different material types.
Determination of the extracellular acidification of Escherichia coli K12 with a multi-​chamber-​based LAPS system (2016)
Dantism, Shahriar ; Takenaga, Shoko ; Wagner, Patrick ; Wagner, Torsten ; Schöning, Michael Josef
On-line monitoring of the metabolic activity of microorganisms involved in intermediate stages of biogas production plays an important role to avoid undesirable “down times” during the biogas production. In order to control this process, an on-chip differential measuring system based on the light-addressable potentiometric sensor (LAPS) principle combined with a 3D-printed multi-chamber structure has been realized. As a test microorganism, Escherichia coli K12 (E. coli K12) were used for cell-based measurements. Multi-chamber structures were developed to determine the metabolic activity of E. coli K12 in suspension for a different number of cells, responding to the addition of a constant or variable amount of glucose concentrations, enabling differential and simultaneous measurements.
Nanocrystalline diamond-based field-effect capacitive pH sensor (2007)
Christiaens, Pieter ; Abouzar, Maryam H. ; Poghossian, Arshak ; Wagner, Torsten ; Bijnens, Nathalie ; Williams, Oliver A. ; Daenen, Michael ; Haenen, Ken ; Schöning, Michael Josef ; Wagner, Patrick
FEM-based modeling of a calorimetric gas sensor for hydrogen peroxide monitoring (2017)
Jildeh, Zaid B. ; Kirchner, Patrick ; Oberländer, Jan ; Kremers, Alexander ; Wagner, Torsten ; Wagner, Patrick H. ; Schöning, Michael Josef
A physically coupled finite element method (FEM) model is developed to study the response behavior of a calorimetric gas sensor. The modeled sensor serves as a monitoring device of the concentration of gaseous hydrogen peroxide (H2 O2) in a high temperature mixture stream in aseptic sterilization processes. The principle of operation of a calorimetric H2 O2 sensor is analyzed and the results of the numerical model have been validated by using previously published sensor experiments. The deviation in the results between the FEM model and experimental data are presented and discussed.
Light-Addressable Actuator-Sensor Platform for Monitoring and Manipulation of pH Gradients in Microfluidics: A Case Study with the Enzyme Penicillinase (2021)
Welden, Rene ; Jablonski, Melanie ; Wege, Christina ; Keusgen, Michael ; Wagner, Patrick Hermann ; Wagner, Torsten ; Schöning, Michael Josef
The feasibility of light-addressed detection and manipulation of pH gradients inside an electrochemical microfluidic cell was studied. Local pH changes, induced by a light-addressable electrode (LAE), were detected using a light-addressable potentiometric sensor (LAPS) with different measurement modes representing an actuator-sensor system. Biosensor functionality was examined depending on locally induced pH gradients with the help of the model enzyme penicillinase, which had been immobilized in the microfluidic channel. The surface morphology of the LAE and enzyme-functionalized LAPS was studied by scanning electron microscopy. Furthermore, the penicillin sensitivity of the LAPS inside the microfluidic channel was determined with regard to the analyte’s pH influence on the enzymatic reaction rate. In a final experiment, the LAE-controlled pH inhibition of the enzyme activity was monitored by the LAPS.
Photoelectrochemical enzymatic penicillin biosensor: A proof-of-concept experiment (2021)
Welden, Rene ; Nagamine Komesu, Cindy A. ; Wagner, Patrick H. ; Schöning, Michael Josef ; Wagner, Torsten
Photoelectrochemical (PEC) biosensors are a rather novel type of biosensors thatutilizelighttoprovideinformationaboutthecompositionofananalyte,enablinglight-controlled multi-analyte measurements. For enzymatic PEC biosensors,amperometric detection principles are already known in the literature. In con-trast, there is only a little information on H+-ion sensitive PEC biosensors. Inthis work, we demonstrate the detection of H+ions emerged by H+-generatingenzymes, exemplarily demonstrated with penicillinase as a model enzyme on atitanium dioxide photoanode. First, we describe the pH sensitivity of the sensorand study possible photoelectrocatalytic reactions with penicillin. Second, weshow the enzymatic PEC detection of penicillin.
A LAPS-based differential sensor for parallelized metabolism monitoring of various bacteria (2019)
Dantism, Shahriar ; Röhlen, Desiree ; Wagner, Torsten ; Wagner, P. ; Schöning, Michael Josef
Monitoring the cellular metabolism of bacteria in (bio)fermentation processes is crucial to control and steer them, and to prevent undesired disturbances linked to metabolically inactive microorganisms. In this context, cell-based biosensors can play an important role to improve the quality and increase the yield of such processes. This work describes the simultaneous analysis of the metabolic behavior of three different types of bacteria by means of a differential light-addressable potentiometric sensor (LAPS) set-up. The study includes Lactobacillus brevis, Corynebacterium glutamicum, and Escherichia coli, which are often applied in fermentation processes in bioreactors. Differential measurements were carried out to compensate undesirable influences such as sensor signal drift, and pH value variation during the measurements. Furthermore, calibration curves of the cellular metabolism were established as a function of the glucose concentration or cell number variation with all three model microorganisms. In this context, simultaneous (bio)sensing with the multi-organism LAPS-based set-up can open new possibilities for a cost-effective, rapid detection of the extracellular acidification of bacteria on a single sensor chip. It can be applied to evaluate the metabolic response of bacteria populations in a (bio)fermentation process, for instance, in the biogas fermentation process.
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