Article
Refine
Year of publication
- 2018 (124) (remove)
Document Type
- Article (124) (remove)
Keywords
- Bahadur efficiency (1)
- Booster Stations (1)
- Buffering Capacity (1)
- Chance Constraint (1)
- Coverage probability (1)
- Cramér-von-Mises statistic (1)
- EBSCO Discovery Service (1)
- Engineering Application (1)
- Equivalence test (1)
- Forschungsprozess (1)
- Goodness-of-fit tests for uniformity (1)
- IBM Watson Explorer (1)
- Integrated empirical distribution (survival) function (1)
- Kernel density estimator (1)
- Lab-on-Chip (1)
- Length of confidence intervals (1)
- Literaturanalyse (1)
- MINLP (1)
- Numerical inversion of Laplace transforms (1)
- Pitman efficiency (1)
Institute
- Fachbereich Medizintechnik und Technomathematik (46)
- IfB - Institut für Bioengineering (22)
- INB - Institut für Nano- und Biotechnologien (19)
- Fachbereich Elektrotechnik und Informationstechnik (13)
- Fachbereich Maschinenbau und Mechatronik (13)
- Fachbereich Chemie und Biotechnologie (12)
- Fachbereich Wirtschaftswissenschaften (12)
- Fachbereich Energietechnik (9)
- Fachbereich Bauingenieurwesen (7)
- Fachbereich Luft- und Raumfahrttechnik (7)
- Fachbereich Architektur (3)
- ECSM European Center for Sustainable Mobility (2)
- Institut fuer Angewandte Polymerchemie (2)
- Nowum-Energy (2)
- Fachbereich Gestaltung (1)
- Solar-Institut Jülich (1)
Cupriavidus necator H16 gains increasing attention in microbial research and biotechnological application due to its diverse metabolic features. Here we present a tightly controlled gene expression system for C. necator including the pBBR1-vector that contains hybrid promoters originating from C. necator native tolC-promoter in combination with a synthetic tetO-operator. The expression of the reporter gene from these plasmids relies on the addition of the exogenous inducer doxycycline (dc). The novel expression system offers a combination of advantageous features as; (i) high and dose-dependent recombinant protein production, (ii) tight control with a high dynamic range (On/Off ratio), which makes it applicable for harmful pathways or for toxic protein production, (iii) comparable cheap inducer (doxycycline, dc), (iv) effective at low inducer concentration, that makes it useful for large scale application, (v) rapid, diffusion controlled induction, and (vi) the inducer does not interfere within the cell metabolism. As applications of the expression system in C. necator H16, the growth ability on glycerol was enhanced by constitutively expressing the E. coli glpk gene-encoding for glycerol kinase. Likewise, we used the system to overcome the expression toxicity of mevalonate pathway in C. necator H16. With this system, the mevalonate-genes were successfully introduced in the host and the recombinant strains could produce about 200 mg/l mevalonate.