Refine
Year of publication
- 2024 (12)
- 2023 (70)
- 2022 (67)
- 2021 (64)
- 2020 (9)
- 2019 (77)
- 2018 (7)
- 2017 (4)
- 2016 (17)
- 2015 (17)
- 2014 (21)
- 2013 (16)
- 2012 (19)
- 2011 (18)
- 2010 (11)
- 2009 (28)
- 2008 (21)
- 2007 (41)
- 2006 (54)
- 2005 (40)
- 2004 (37)
- 2003 (36)
- 2002 (18)
- 2001 (11)
- 2000 (11)
- 1999 (4)
- 1998 (6)
- 1997 (6)
- 1996 (2)
- 1989 (1)
- 1988 (1)
- 1985 (1)
- 1925 (1)
Document Type
- Conference Proceeding (227)
- Bachelor Thesis (141)
- Article (122)
- Administrative publication (76)
- Part of Periodical (61)
- Lecture (30)
- Book (22)
- Report (19)
- Diploma Thesis (12)
- Course Material (9)
- Other (9)
- Study Thesis (5)
- Working Paper (5)
- Part of a Book (4)
- Master's Thesis (3)
- Talk (2)
- Poster (1)
Language
- German (522)
- English (218)
- Portuguese (6)
- Multiple languages (1)
- Spanish (1)
Has Fulltext
- yes (748) (remove)
Keywords
- Amtliche Mitteilung (71)
- Bachelor (33)
- Aachen University of Applied Sciences (31)
- Master (31)
- Prüfungsordnung (31)
- Bauingenieurwesen (30)
- Lesbare Fassung (28)
- Biosensor (25)
- Fachhochschule Aachen (23)
- Studien- und Prüfungsordnung (21)
- Aachen / Fachhochschule Aachen (20)
- Änderungsordnung (20)
- Blitzschutz (18)
- Elektromobilität (17)
- CAD (16)
- Finite-Elemente-Methode (16)
- civil engineering (14)
- Einspielen <Werkstoff> (13)
- Illustration (12)
- Elektrofahrzeug (11)
Institute
- Fachbereich Gestaltung (151)
- FH Aachen (150)
- Fachbereich Medizintechnik und Technomathematik (145)
- Fachbereich Maschinenbau und Mechatronik (67)
- IfB - Institut für Bioengineering (63)
- Fachbereich Energietechnik (48)
- Fachbereich Bauingenieurwesen (47)
- Fachbereich Wirtschaftswissenschaften (38)
- Fachbereich Elektrotechnik und Informationstechnik (34)
- INB - Institut für Nano- und Biotechnologien (29)
- Fachbereich Luft- und Raumfahrttechnik (27)
- Fachbereich Chemie und Biotechnologie (18)
- Fachbereich Architektur (13)
- ZHQ - Bereich Hochschuldidaktik und Evaluation (12)
- ECSM European Center for Sustainable Mobility (6)
- Nowum-Energy (6)
- MASKOR Institut für Mobile Autonome Systeme und Kognitive Robotik (5)
- Solar-Institut Jülich (5)
- Arbeitsstelle fuer Hochschuldidaktik und Studienberatung (1)
- IBB - Institut für Baustoffe und Baukonstruktionen (1)
Halophilic and halotolerant microorganisms represent a promising source of salt-tolerant enzymes suitable for various biotechnological applications where high salt concentrations would otherwise limit enzymatic activity. Considering the current growing enzyme market and the need for more efficient and new biocatalysts, the present study aimed at the characterization of a high-alkaline subtilisin from Alkalihalobacillus okhensis Kh10-101T. The protease gene was cloned and expressed in Bacillus subtilis DB104. The recombinant protease SPAO with 269 amino acids belongs to the subfamily of high-alkaline subtilisins. The biochemical characteristics of purified SPAO were analyzed in comparison with subtilisin Carlsberg, Savinase, and BPN'. SPAO, a monomer with a molecular mass of 27.1 kDa, was active over a wide range of pH 6.0–12.0 and temperature 20–80 °C, optimally at pH 9.0–9.5 and 55 °C. The protease is highly oxidatively stable to hydrogen peroxide and retained 58% of residual activity when incubated at 10 °C with 5% (v/v) H2O2 for 1 h while stimulated at 1% (v/v) H2O2. Furthermore, SPAO was very stable and active at NaCl concentrations up to 5.0 m. This study demonstrates the potential of SPAO for biotechnological applications in the future.