Refine
Year of publication
- 2020 (232) (remove)
Institute
- Fachbereich Medizintechnik und Technomathematik (59)
- Fachbereich Energietechnik (35)
- Fachbereich Wirtschaftswissenschaften (34)
- IfB - Institut für Bioengineering (32)
- Fachbereich Luft- und Raumfahrttechnik (31)
- Fachbereich Elektrotechnik und Informationstechnik (29)
- ECSM European Center for Sustainable Mobility (17)
- Fachbereich Maschinenbau und Mechatronik (16)
- Fachbereich Bauingenieurwesen (14)
- Solar-Institut Jülich (14)
Has Fulltext
- no (232) (remove)
Language
- English (168)
- German (62)
- Multiple languages (1)
- Dutch (1)
Document Type
- Article (127)
- Conference Proceeding (55)
- Part of a Book (19)
- Book (9)
- Review (7)
- Other (4)
- Doctoral Thesis (3)
- Patent (3)
- Conference Poster (2)
- Conference: Meeting Abstract (1)
Keywords
- MINLP (3)
- Additive manufacturing (2)
- Adjacent buildings (2)
- Experimental validation (2)
- Historical centres (2)
- INODIS (2)
- Shake table test (2)
- Stone masonry (2)
- rebound-effect (2)
- sustainability (2)
Is part of the Bibliography
- no (232)
Multi-enzyme immobilization onto a capacitive field-effect biosensor by nano-spotting technique is presented. The nano-spotting technique allows to immobilize different enzymes simultaneously on the sensor surface with high spatial resolution without additional photolithographical patterning. The amount of applied enzymatic cocktail on the sensor surface can be tailored. Capacitive electrolyte-insulator-semiconductor (EIS) field-effect sensors with Ta2O5 as pH-sensitive transducer layer have been chosen to immobilize the three different (pL droplets) enzymes penicillinase, urease, and glucose oxidase. Nano-spotting immobilization is compared to conventional drop-coating method by defining different geometrical layouts on the sensor surface (fully, half-, and quarter-spotted). The drop diameter is varying between 84 µm and 102 µm, depending on the number of applied drops (1 to 4) per spot. For multi-analyte detection, penicillinase and urease are simultaneously nano-spotted on the EIS sensor. Sensor characterization was performed by C/V (capacitance/voltage) and ConCap (constant capacitance) measurements. Average penicillin, glucose, and urea sensitivities for the spotted enzymes were 81.7 mV/dec, 40.5 mV/dec, and 68.9 mV/dec, respectively.