Refine
Year of publication
- 2023 (28) (remove)
Document Type
- Article (22)
- Conference Proceeding (4)
- Habilitation (1)
- Preprint (1)
Language
- English (28) (remove)
Keywords
- Information extraction (3)
- Natural language processing (3)
- ultrasound (2)
- Active learning (1)
- Agent-based simulation (1)
- Architectural design (1)
- Asymptotic relative efficiency (1)
- Bacillus atrophaeus spores (1)
- Bacterial cellulose (1)
- Bioabsorbable (1)
- Capacitive field-effect sensor (1)
- Carbon sources (1)
- Cellulose nanostructure (1)
- Clustering (1)
- Competitiveness (1)
- Conductive Boundary Condition (1)
- Cost-effectiveness (1)
- Cramér-von-Mises test (1)
- Cross border adjustment mechanism (1)
- Culture media (1)
- DPA (dipicolinic acid) (1)
- Deep learning (1)
- E-Mobility (1)
- Endothelial dysfunction (1)
- Energy market design (1)
- Energy-intensive industry (1)
- Enterprise information systems (1)
- Fault approximation (1)
- Fault detection (1)
- Floor prices (1)
- Geriatric (1)
- Gold nanoparticles (1)
- Hip fractures (1)
- Incomplete data (1)
- Inverse Scattering (1)
- Inverse scattering problem (1)
- Label-free detection (1)
- LbL films (1)
- Long COVID (1)
- MCDA (1)
- Marginal homogeneity (1)
- Market modeling (1)
- Medusomyces gisevi (1)
- Mobility transition (1)
- Model-driven software engineering (1)
- Multi-criteria decision analysis (1)
- Multicell (1)
- Multiplexing (1)
- Natural language understanding (1)
- Paired sample (1)
- Polylactide acid (1)
- Post-COVID-19 syndrome (1)
- Preference assessment (1)
- Prevention (1)
- Profile extraction (1)
- Prophylaxis (1)
- Query learning (1)
- Raman spectroscopy (1)
- Regionalization (1)
- Relation classification (1)
- Reproducible research (1)
- Resistive temperature detector (1)
- Silk fibroin (1)
- Sn₃O₄ (1)
- Software and systems modeling (1)
- Steel industry (1)
- Text mining (1)
- Transmission Eigenvalues (1)
- Trustworthy artificial intelligence (1)
- Volumes of confidence regions (1)
- allocation (1)
- amperometric biosensors (1)
- biocompatible (1)
- biodegradabl (1)
- biomechanics (1)
- biosensor (1)
- central symmetry test (1)
- conditional excess distribution (1)
- conditional expectation principle (1)
- confidence interval (1)
- connective tissue (1)
- covariance principle (1)
- electromyography (1)
- encapsulation materials (1)
- enzyme cascade (1)
- exchangeability test (1)
- fibroin (1)
- field-effect sensor (1)
- forecast (1)
- glucose oxidase (GOx) (1)
- goodness-of-fit test (1)
- heavy metals (1)
- horseradish peroxidase (HRP) (1)
- independence test (1)
- locomotion (1)
- nanobelts (1)
- not identically distributed (1)
- optical sensor setup (1)
- optical trapping (1)
- overload (1)
- physiology (1)
- portfolio risk (1)
- random effects (1)
- retinal microvasculature (1)
- sterilization (1)
- stretch-shortening cycle (1)
- tobacco mosaic virus (TMV) (1)
- turnip vein clearing virus (TVCV) (1)
Institute
- Fachbereich Medizintechnik und Technomathematik (28) (remove)
Hydrogen peroxide (H₂O₂), a strong oxidizer, is a commonly used sterilization agent employed during aseptic food processing and medical applications. To assess the sterilization efficiency with H₂O₂, bacterial spores are common microbial systems due to their remarkable robustness against a wide variety of decontamination strategies. Despite their widespread use, there is, however, only little information about the detailed time-resolved mechanism underlying the oxidative spore death by H₂O₂. In this work, we investigate chemical and morphological changes of individual Bacillus atrophaeus spores undergoing oxidative damage using optical sensing with trapping Raman microscopy in real-time. The time-resolved experiments reveal that spore death involves two distinct phases: (i) an initial phase dominated by the fast release of dipicolinic acid (DPA), a major spore biomarker, which indicates the rupture of the spore’s core; and (ii) the oxidation of the remaining spore material resulting in the subsequent fragmentation of the spores’ coat. Simultaneous observation of the spore morphology by optical microscopy corroborates these mechanisms. The dependence of the onset of DPA release and the time constant of spore fragmentation on H₂O₂ shows that the formation of reactive oxygen species from H₂O₂ is the rate-limiting factor of oxidative spore death.