Article
Refine
Year of publication
- 2024 (5) (remove)
Document Type
- Article (5) (remove)
Language
- English (5) (remove)
Keywords
- Acyl-amino acids (1)
- Acylation (1)
- Aminoacylase (1)
- Biocatalysis (1)
- Biosurfactants (1)
- Capacitive model (1)
- Chaperone (1)
- Enzyme coverage (1)
- Field-effect biosensor (1)
- MOS (1)
- Multianalyte detection (1)
- Penicillin (1)
- Raman spectroscopy (1)
- artificial olfactory image (1)
- catalytic metal (1)
- coupled Néel–Brownian relaxation dynamics (1)
- electronic nose (1)
- field-effect structure (1)
- frequency mixing magnetic detection (1)
- gas sensor (1)
Institute
- INB - Institut für Nano- und Biotechnologien (5) (remove)
Electrolyte-insulator-semiconductor capacitors (EISCAP) belong to field-effect sensors having an attractive transducer architecture for constructing various biochemical sensors. In this study, a capacitive model of enzyme-modified EISCAPs has been developed and the impact of the surface coverage of immobilized enzymes on its capacitance-voltage and constant-capacitance characteristics was studied theoretically and experimentally. The used multicell arrangement enables a multiplexed electrochemical characterization of up to sixteen EISCAPs. Different enzyme coverages have been achieved by means of parallel electrical connection of bare and enzyme-covered single EISCAPs in diverse combinations. As predicted by the model, with increasing the enzyme coverage, both the shift of capacitance-voltage curves and the amplitude of the constant-capacitance signal increase, resulting in an enhancement of analyte sensitivity of the EISCAP biosensor. In addition, the capability of the multicell arrangement with multi-enzyme covered EISCAPs for sequentially detecting multianalytes (penicillin and urea) utilizing the enzymes penicillinase and urease has been experimentally demonstrated and discussed.