Article
Refine
Year of publication
Document Type
- Article (46) (remove)
Language
- English (46) (remove)
Keywords
- biosensors (3)
- capacitive field-effect sensor (2)
- tobacco mosaic virus (TMV) (2)
- Bacillus atrophaeus (1)
- Bioabsorbable (1)
- Calorimetric gas sensor (1)
- Capacitive field-effect sensor (1)
- Dehydrogenase (1)
- Diaphorase (1)
- Enzymatic biosensor (1)
- Hydrogen peroxide (1)
- Label-free detection (1)
- O2 plasma (1)
- Plant virus (1)
- Polyimide (1)
- Polylactide acid (1)
- Resistive temperature detector (1)
- Silk fibroin (1)
- Simultaneous determination (1)
- Sterilisation process (1)
Institute
- INB - Institut für Nano- und Biotechnologien (46) (remove)
A capacitive electrolyte-insulator-semiconductor (EIS) field-effect biosensor for acetoin detection has been presented for the first time. The EIS sensor consists of a layer structure of Al/p-Si/SiO₂/Ta₂O₅/enzyme acetoin reductase. The enzyme, also referred to as butane-2,3-diol dehydrogenase from B. clausii DSM 8716T, has been recently characterized. The enzyme catalyzes the (R)-specific reduction of racemic acetoin to (R,R)- and meso-butane-2,3-diol, respectively. Two different enzyme immobilization strategies (cross-linking by using glutaraldehyde and adsorption) have been studied. Typical biosensor parameters such as optimal pH working range, sensitivity, hysteresis, linear concentration range and long-term stability have been examined by means of constant-capacitance (ConCap) mode measurements. Furthermore, preliminary experiments have been successfully carried out for the detection of acetoin in diluted white wine samples.