Refine
Year of publication
Document Type
- Article (150)
- Conference Proceeding (18)
- Part of a Book (7)
- Book (1)
Language
- English (176) (remove)
Keywords
- Label-free detection (3)
- capacitive field-effect sensor (3)
- tobacco mosaic virus (TMV) (3)
- Biosensor (2)
- Capacitive field-effect sensor (2)
- Tobacco mosaic virus (TMV) (2)
- field-effect sensor (2)
- gold nanoparticles (2)
- (Bio)degradation (1)
- Biosensorik (1)
- CNOT (1)
- Capacitive field-effect (1)
- Capacitive model (1)
- Chemical imaging (1)
- Coat protein (1)
- C–V method (1)
- DNA biosensor (1)
- Electrolyte–insulator–semiconductor (1)
- Enzyme coverage (1)
- Enzyme logic gate (1)
Institute
- INB - Institut für Nano- und Biotechnologien (176) (remove)
The coupling of ligand-stabilized gold nanoparticles with field-effect devices offers new possibilities for label-free biosensing. In this work, we study the immobilization of aminooctanethiol-stabilized gold nanoparticles (AuAOTs) on the silicon dioxide surface of a capacitive field-effect sensor. The terminal amino group of the AuAOT is well suited for the functionalization with biomolecules. The attachment of the positively-charged AuAOTs on a capacitive field-effect sensor was detected by direct electrical readout using capacitance-voltage and constant capacitance measurements. With a higher particle density on the sensor surface, the measured signal change was correspondingly more pronounced. The results demonstrate the ability of capacitive field-effect sensors for the non-destructive quantitative validation of nanoparticle immobilization. In addition, the electrostatic binding of the polyanion polystyrene sulfonate to the AuAOT-modified sensor surface was studied as a model system for the label-free detection of charged macromolecules. Most likely, this approach can be transferred to the label-free detection of other charged molecules such as enzymes or antibodies.