Article
Refine
Year of publication
- 2018 (125) (remove)
Institute
- Fachbereich Medizintechnik und Technomathematik (47)
- IfB - Institut für Bioengineering (22)
- INB - Institut für Nano- und Biotechnologien (19)
- Fachbereich Elektrotechnik und Informationstechnik (13)
- Fachbereich Maschinenbau und Mechatronik (13)
- Fachbereich Chemie und Biotechnologie (12)
- Fachbereich Wirtschaftswissenschaften (12)
- Fachbereich Energietechnik (9)
- Fachbereich Bauingenieurwesen (7)
- Fachbereich Luft- und Raumfahrttechnik (7)
Has Fulltext
- no (125)
Document Type
- Article (125) (remove)
Keywords
- Bahadur efficiency (1)
- Booster Stations (1)
- Buffering Capacity (1)
- Chance Constraint (1)
- Conductive boundary condition (1)
- Coverage probability (1)
- Cramér-von-Mises statistic (1)
- EBSCO Discovery Service (1)
- Engineering Application (1)
- Equivalence test (1)
- Forschungsprozess (1)
- Goodness-of-fit tests for uniformity (1)
- IBM Watson Explorer (1)
- Integrated empirical distribution (survival) function (1)
- Inverse scattering (1)
- Kernel density estimator (1)
- Lab-on-Chip (1)
- Length of confidence intervals (1)
- Literaturanalyse (1)
- MINLP (1)
- Numerical inversion of Laplace transforms (1)
- Pitman efficiency (1)
- Pump System (1)
- Rehabilitation Technology and Prosthetics (1)
- Stochastic Programming (1)
- Surgical Navigation and Robotics (1)
- Text Analytics (1)
- Transmission eigenvalues (1)
- Uncertainty (1)
- Water Distribution (1)
- Water Supply Networks (1)
- Wilcoxon tests (1)
- achilles tendon (1)
- design of technical systems (1)
- energy absorption (1)
- energy dissipation (1)
- frequency mixing (1)
- functional data (1)
- huge dimensional data (1)
- legal obligations (1)
- magnetic beads (1)
- magnetic sensing (1)
- mathematical optimization (1)
- mechanical buffer (1)
- optimization (1)
- product liability (1)
- remote sensing (1)
- resilience (1)
- separable Hilbert space (1)
- slum classification (1)
- stiffness (1)
- superparamagnetic nanoparticles (1)
- water supply design (1)
Is part of the Bibliography
- no (125)
Cupriavidus necator H16 gains increasing attention in microbial research and biotechnological application due to its diverse metabolic features. Here we present a tightly controlled gene expression system for C. necator including the pBBR1-vector that contains hybrid promoters originating from C. necator native tolC-promoter in combination with a synthetic tetO-operator. The expression of the reporter gene from these plasmids relies on the addition of the exogenous inducer doxycycline (dc). The novel expression system offers a combination of advantageous features as; (i) high and dose-dependent recombinant protein production, (ii) tight control with a high dynamic range (On/Off ratio), which makes it applicable for harmful pathways or for toxic protein production, (iii) comparable cheap inducer (doxycycline, dc), (iv) effective at low inducer concentration, that makes it useful for large scale application, (v) rapid, diffusion controlled induction, and (vi) the inducer does not interfere within the cell metabolism. As applications of the expression system in C. necator H16, the growth ability on glycerol was enhanced by constitutively expressing the E. coli glpk gene-encoding for glycerol kinase. Likewise, we used the system to overcome the expression toxicity of mevalonate pathway in C. necator H16. With this system, the mevalonate-genes were successfully introduced in the host and the recombinant strains could produce about 200 mg/l mevalonate.