Refine
Year of publication
- 2024 (23) (remove)
Institute
- Fachbereich Medizintechnik und Technomathematik (23) (remove)
Language
- English (23) (remove)
Document Type
- Article (16)
- Conference Proceeding (5)
- Book (1)
- Preprint (1)
Keywords
- Ageing (1)
- Agent-based modeling (1)
- Analytical models (1)
- Anatomy (1)
- Boundary integral equations, (1)
- Capacitive model (1)
- Coal (1)
- Comparative simulation (1)
- Complex-valued eigenvalues (1)
- Constitutive model (1)
- Damage mechanics theory (1)
- Database (1)
- Discontinuous fractures (1)
- E. coli detection (1)
- Eigenvalue trajectories (1)
- Energy dispatch (1)
- Energy market (1)
- Enzyme coverage (1)
- Exponential Euler scheme, (1)
- Field-effect biosensor (1)
- Focusing (1)
- German (1)
- H2 (1)
- Hydrogenotrophic methanogens (1)
- Instruments (1)
- Interior transmission problem (1)
- MOS (1)
- Master stamp (1)
- Measurement (1)
- Methane (1)
- Methanogenesis (1)
- Multianalyte detection (1)
- Muscle (1)
- Musculoskeletal system (1)
- Non-parallel fissures (1)
- Nonlinear eigenvalue problems (1)
- Open Data (1)
- Open source (1)
- Parabolic SPDEs (1)
- Penicillin (1)
- Photolithographic mimics (1)
- Physiology (1)
- Quartz crystal microbalance (1)
- Raman spectroscopy (1)
- Refining (1)
- Renewable energy sources (1)
- Simulation (1)
- Surface imprinted polymer (1)
- Time-series (1)
- Uniaxial compression test (1)
- aquaculture (1)
- artificial olfactory image (1)
- catalytic metal (1)
- chip-based sensor setup (1)
- coupled Néel–Brownian relaxation dynamics (1)
- electrical conductivity of liquids (1)
- electronic nose (1)
- field-effect structure (1)
- frequency mixing magnetic detection (1)
- gas sensor (1)
- hydrogen peroxide (1)
- impedance spectroscopy (1)
- key performance indicators (1)
- large language models (1)
- light-addressing technologies (1)
- magnetic biosensing (1)
- magnetic nanoparticles (1)
- magnetic relaxation (1)
- metal-oxide-semiconductor structure (1)
- microfluidics (1)
- micromagnetic simulation (1)
- optical spore trapping (1)
- scanned light pulse technique (1)
- semantic role labeling (1)
- speaker attribution (1)
- sterilization conditions (1)
- temperature (1)
- thermometry (1)
- visualization (1)
To gain insight on chemical sterilization processes, the influence of temperature (up to 70 °C), intense green light, and hydrogen peroxide (H₂O₂) concentration (up to 30% in aqueous solution) on microbial spore inactivation is evaluated by in-situ Raman spectroscopy with an optical trap. Bacillus atrophaeus is utilized as a model organism. Individual spores are isolated and their chemical makeup is monitored under dynamically changing conditions (temperature, light, and H₂O₂ concentration) to mimic industrially relevant process parameters for sterilization in the field of aseptic food processing. While isolated spores in water are highly stable, even at elevated temperatures of 70 °C, exposure to H₂O₂ leads to a loss of spore integrity characterized by the release of the key spore biomarker dipicolinic acid (DPA) in a concentration-dependent manner, which indicates damage to the inner membrane of the spore. Intensive light or heat, both of which accelerate the decomposition of H₂O₂ into reactive oxygen species (ROS), drastically shorten the spore lifetime, suggesting the formation of ROS as a rate-limiting step during sterilization. It is concluded that Raman spectroscopy can deliver mechanistic insight into the mode of action of H₂O₂-based sterilization and reveal the individual contributions of different sterilization methods acting in tandem.