Refine
Year of publication
Institute
- Fachbereich Medizintechnik und Technomathematik (2074)
- Fachbereich Elektrotechnik und Informationstechnik (1184)
- Fachbereich Wirtschaftswissenschaften (1158)
- Fachbereich Energietechnik (1115)
- Fachbereich Chemie und Biotechnologie (912)
- Fachbereich Maschinenbau und Mechatronik (868)
- Fachbereich Luft- und Raumfahrttechnik (798)
- Fachbereich Bauingenieurwesen (711)
- IfB - Institut für Bioengineering (690)
- INB - Institut für Nano- und Biotechnologien (615)
Language
- German (5114)
- English (4909)
- Russian (14)
- Portuguese (6)
- Multiple languages (5)
- Spanish (3)
- Dutch (2)
- Italian (1)
Document Type
- Article (5646)
- Conference Proceeding (1639)
- Book (1079)
- Part of a Book (559)
- Bachelor Thesis (327)
- Patent (174)
- Report (101)
- Doctoral Thesis (79)
- Other (76)
- Administrative publication (76)
- Conference: Meeting Abstract (75)
- Part of a Periodical (63)
- Lecture (30)
- Contribution to a Periodical (20)
- Master's Thesis (20)
- Review (18)
- Working Paper (18)
- Diploma Thesis (15)
- Course Material (9)
- Talk (7)
- Conference Poster (6)
- Habilitation (5)
- Preprint (5)
- Study Thesis (5)
- Examination Thesis (1)
- Video (1)
Keywords
- Amtliche Mitteilung (71)
- Bachelor (33)
- Aachen University of Applied Sciences (31)
- Master (31)
- Prüfungsordnung (31)
- Bauingenieurwesen (30)
- Lesbare Fassung (28)
- Biosensor (25)
- Fachhochschule Aachen (23)
- Illustration (23)
Zugriffsart
- campus (2147)
- weltweit (1860)
- bezahl (766)
- fachbereichsweit (FB4) (34)
To gain insight on chemical sterilization processes, the influence of temperature (up to 70 °C), intense green light, and hydrogen peroxide (H₂O₂) concentration (up to 30% in aqueous solution) on microbial spore inactivation is evaluated by in-situ Raman spectroscopy with an optical trap. Bacillus atrophaeus is utilized as a model organism. Individual spores are isolated and their chemical makeup is monitored under dynamically changing conditions (temperature, light, and H₂O₂ concentration) to mimic industrially relevant process parameters for sterilization in the field of aseptic food processing. While isolated spores in water are highly stable, even at elevated temperatures of 70 °C, exposure to H₂O₂ leads to a loss of spore integrity characterized by the release of the key spore biomarker dipicolinic acid (DPA) in a concentration-dependent manner, which indicates damage to the inner membrane of the spore. Intensive light or heat, both of which accelerate the decomposition of H₂O₂ into reactive oxygen species (ROS), drastically shorten the spore lifetime, suggesting the formation of ROS as a rate-limiting step during sterilization. It is concluded that Raman spectroscopy can deliver mechanistic insight into the mode of action of H₂O₂-based sterilization and reveal the individual contributions of different sterilization methods acting in tandem.