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- 2024 (3) (remove)
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- Capacitive model (1)
- Enzyme coverage (1)
- Field-effect biosensor (1)
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- Multianalyte detection (1)
- Penicillin (1)
- Raman spectroscopy (1)
- artificial olfactory image (1)
- catalytic metal (1)
- electronic nose (1)
- field-effect structure (1)
- gas sensor (1)
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- metal-oxide-semiconductor structure (1)
- optical spore trapping (1)
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Electrolyte-insulator-semiconductor capacitors (EISCAP) belong to field-effect sensors having an attractive transducer architecture for constructing various biochemical sensors. In this study, a capacitive model of enzyme-modified EISCAPs has been developed and the impact of the surface coverage of immobilized enzymes on its capacitance-voltage and constant-capacitance characteristics was studied theoretically and experimentally. The used multicell arrangement enables a multiplexed electrochemical characterization of up to sixteen EISCAPs. Different enzyme coverages have been achieved by means of parallel electrical connection of bare and enzyme-covered single EISCAPs in diverse combinations. As predicted by the model, with increasing the enzyme coverage, both the shift of capacitance-voltage curves and the amplitude of the constant-capacitance signal increase, resulting in an enhancement of analyte sensitivity of the EISCAP biosensor. In addition, the capability of the multicell arrangement with multi-enzyme covered EISCAPs for sequentially detecting multianalytes (penicillin and urea) utilizing the enzymes penicillinase and urease has been experimentally demonstrated and discussed.