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Institute
- Fachbereich Medizintechnik und Technomathematik (9)
- INB - Institut für Nano- und Biotechnologien (5)
- IfB - Institut für Bioengineering (3)
- Fachbereich Chemie und Biotechnologie (2)
- Fachbereich Elektrotechnik und Informationstechnik (2)
- Fachbereich Luft- und Raumfahrttechnik (2)
- ECSM European Center for Sustainable Mobility (1)
- Institut fuer Angewandte Polymerchemie (1)
Electrolyte-insulator-semiconductor capacitors (EISCAP) belong to field-effect sensors having an attractive transducer architecture for constructing various biochemical sensors. In this study, a capacitive model of enzyme-modified EISCAPs has been developed and the impact of the surface coverage of immobilized enzymes on its capacitance-voltage and constant-capacitance characteristics was studied theoretically and experimentally. The used multicell arrangement enables a multiplexed electrochemical characterization of up to sixteen EISCAPs. Different enzyme coverages have been achieved by means of parallel electrical connection of bare and enzyme-covered single EISCAPs in diverse combinations. As predicted by the model, with increasing the enzyme coverage, both the shift of capacitance-voltage curves and the amplitude of the constant-capacitance signal increase, resulting in an enhancement of analyte sensitivity of the EISCAP biosensor. In addition, the capability of the multicell arrangement with multi-enzyme covered EISCAPs for sequentially detecting multianalytes (penicillin and urea) utilizing the enzymes penicillinase and urease has been experimentally demonstrated and discussed.