Refine
Year of publication
- 2017 (155) (remove)
Institute
- Fachbereich Medizintechnik und Technomathematik (61)
- IfB - Institut für Bioengineering (32)
- INB - Institut für Nano- und Biotechnologien (26)
- Fachbereich Elektrotechnik und Informationstechnik (25)
- Fachbereich Luft- und Raumfahrttechnik (22)
- Fachbereich Energietechnik (14)
- Fachbereich Chemie und Biotechnologie (12)
- Fachbereich Maschinenbau und Mechatronik (10)
- MASKOR Institut für Mobile Autonome Systeme und Kognitive Robotik (10)
- Fachbereich Bauingenieurwesen (7)
Language
- English (155) (remove)
Document Type
- Article (72)
- Conference Proceeding (67)
- Part of a Book (12)
- Other (2)
- Book (1)
- Patent (1)
Keywords
- MASCOT (2)
- 3D nonlinear finite element model (1)
- Acceptance tests (1)
- Automated Optimization (1)
- Autonomous mobile robots (1)
- Biomolecular logic gate (1)
- CNOT (1)
- Capacitive field-effect (1)
- Chemical images (1)
- Chemical sensor (1)
- Chimeric liver-humanized mice (1)
- Competence Developing Games (1)
- DNA (1)
- Dehydrogenase (1)
- Diaphorase (1)
- Diversity (1)
- Diversity Management (1)
- Drug distribution (1)
- Drug metabolism (1)
- Dry-low-NOx (DLN) combustion (1)
- EIS capacitive sensor (1)
- Effective modal mass (1)
- Electrolyte–insulator–semiconductor (1)
- Enterprise architecture (1)
- Enterprise transformation (1)
- Enzymatic biosensor (1)
- Enzyme biosensor (1)
- Enzyme logic gate (1)
- Field-effect sensor (1)
- GOSSAMER-1 (1)
- Gamification (1)
- Gold nanoparticle (1)
- Gossamer (1)
- High hydrogen combustion (1)
- Higher education (1)
- Hotelling’s T² test (1)
- Hydrogen combustion (1)
- Hydrogen gas turbine (1)
- Image Database (1)
- Image Forensics (1)
- Industry 4.0 (1)
- Innovation (1)
- Innovation Management (1)
- Knockout mice (1)
- LAPS (1)
- Leadership (1)
- Light-addressable potentiometric sensor (1)
- Map (eTOM) Process reference model Process design Telecommunications industry (1)
- Micromix combustion (1)
- Mixed-Methods Research (1)
- Mobile Phones (1)
- Multi-robot systems (1)
- Multiphase (1)
- Natural frequency (1)
- Negative impedance convertor (1)
- O2 plasma (1)
- Optimal Topology (1)
- PHILAE (1)
- Performance measurement (1)
- Piecewise Linearization (1)
- Plant efficiency (1)
- Process design (1)
- Process reference model (1)
- Quantitative research (1)
- Reference modelling (1)
- Resonance-mode measurement (1)
- RoboCup (1)
- Sequence-Search (1)
- Serious Games (1)
- Similarity Theory (1)
- Simultaneous determination (1)
- Small Solar System Body Lander (1)
- Small Spacecraft (1)
- Smart factory (1)
- Solar Power Sail (1)
- Students (1)
- Suspension bridge (1)
- TM Forum (1)
- Telecommunication (1)
- Thin shell finite elements (1)
- Toxicology (1)
- Trajectory Optimization (1)
- Transition (1)
- Ventilation System (1)
- XOR (1)
- annealing (1)
- asteroid sample return (1)
- change management (1)
- churches (1)
- collaboration of architects and structural engineers (1)
- complete block symmetry (1)
- diversity management (1)
- earthquake engineering (1)
- education (1)
- elastomeric bearing (1)
- electrolyte-insulator semiconductor sensor (EIS) (1)
- engineering (1)
- enhanced Telecom Operations Map (eTOM) (1)
- equivalent stiffness (1)
- friction pendulum bearing (1)
- granular silo (1)
- hydroxylation (1)
- hypoplasticity (1)
- innovation management (1)
- interdisciplinary student projects (1)
- learning theories (1)
- likelihood ratio test (1)
- liquid storage tank (1)
- macro-element (1)
- motivation theories (1)
- multiple NEA rendezvous (1)
- nonlinear transient analyses (1)
- perception of structures (1)
- research association (1)
- seismic isolation (1)
- simplified approach (1)
- solar sail (1)
- structural systems (1)
- surface functionalization (1)
- teaching of structures (1)
- truss (1)
- uniformly most powerful invariant test (1)
- vault (1)
Is part of the Bibliography
- no (155)
The incorporation of nanomaterials that are biocompatible with different types of biological compounds has allowed the development of a new generation of biosensors applied especially in the biomedical field. In particular, the integration of film-based nanomaterials employed in field-effect devices can be interesting to develop biosensors with enhanced properties. In this paper, we studied the fabrication of sensitive nanofilms combining ZnO nanocrystals and carbon nanotubes (CNTs), prepared by means of the layer-by-layer (LbL) technique, in a capacitive electrolyte-insulator-semiconductor (EIS) structure for detecting glucose and urea. The ZnO nanocrystals were incorporated in a polymeric matrix of poly(allylamine) hydrochloride (PAH), and arranged with multi-walled CNTs in a LbL PAH-ZnO/CNTs film architecture onto EIS chips. The electrochemical characterizations were performed by capacitance–voltage and constant capacitance measurements, while the morphology of the films was characterized by atomic force microscopy. The enzymes glucose oxidase and urease were immobilized on film’s surface for detection of glucose and urea, respectively. In order to obtain glucose and urea biosensors with optimized amount of sensitive films, we investigated the ideal number of bilayers for each detection system. The glucose biosensor showed better sensitivity and output signal for an LbL PAH-ZnO/CNTs nanofilm with 10 bilayers. On the other hand, the urea biosensor presented enhanced properties even for the first bilayer, exhibiting high sensitivity and output signal. The presence of the LbL PAH-ZnO/CNTs films led to biosensors with better sensitivity and enhanced response signal, demonstrating that the adequate use of nanostructured films is feasible for proof-of-concept biosensors with improved properties that may be employed for biomedical applications.
Three amperometric biosensors have been developed for the detection of L-malic acid, fumaric acid, and L -aspartic acid, all based on the combination of a malate-specific dehydrogenase (MDH, EC 1.1.1.37) and diaphorase (DIA, EC 1.8.1.4). The stepwise expansion of the malate platform with the enzymes fumarate hydratase (FH, EC 4.2.1.2) and aspartate ammonia-lyase (ASPA, EC 4.3.1.1) resulted in multi-enzyme reaction cascades and, thus, augmentation of the substrate spectrum of the sensors. Electrochemical measurements were carried out in presence of the cofactor β-nicotinamide adenine dinucleotide (NAD+) and the redox mediator hexacyanoferrate (III) (HCFIII). The amperometric detection is mediated by oxidation of hexacyanoferrate (II) (HCFII) at an applied potential of + 0.3 V vs. Ag/AgCl. For each biosensor, optimum working conditions were defined by adjustment of cofactor concentrations, buffer pH, and immobilization procedure. Under these improved conditions, amperometric responses were linear up to 3.0 mM for L-malate and fumarate, respectively, with a corresponding sensitivity of 0.7 μA mM−1 (L-malate biosensor) and 0.4 μA mM−1 (fumarate biosensor). The L-aspartate detection system displayed a linear range of 1.0–10.0 mM with a sensitivity of 0.09 μA mM−1. The sensor characteristics suggest that the developed platform provides a promising method for the detection and differentiation of the three substrates.
The immobilization of NAD+-dependent dehydrogenases, in combination with a diaphorase, enables the facile development of multiparametric sensing devices. In this work, an amperometric biosensor array for simultaneous determination of ethanol, formate, d- and l-lactate is presented. Enzyme immobilization on platinum thin-film electrodes was realized by chemical cross-linking with glutaraldehyde. The optimization of the sensor performance was investigated with regard to enzyme loading, glutaraldehyde concentration, pH, cofactor concentration and temperature. Under optimal working conditions (potassium phosphate buffer with pH 7.5, 2.5 mmol L-1 NAD+, 2.0 mmol L-1 ferricyanide, 25 °C and 0.4% glutaraldehyde) the linear working range and sensitivity of the four sensor elements was improved. Simultaneous and cross-talk free measurements of four different metabolic parameters were performed successfully. The reliable analytical performance of the biosensor array was demonstrated by application in a clarified sample of inoculum sludge. Thereby, a promising approach for on-site monitoring of fermentation processes is provided.