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Is part of the Bibliography
- no (258)
The conjunction of (bio-)chemical recognition elements with nanoscale biological building blocks such as virus particles is considered as a very promising strategy for the creation of biohybrids opening novel opportunities for label-free biosensing. This work presents a new approach for the development of biosensors using tobacco mosaic virus (TMV) nanotubes or coat proteins (CPs) as enzyme nanocarriers. Sensor chips combining an array of Pt electrodes loaded with glucose oxidase (GOD)-modified TMV nanotubes or CP aggregates were used for amperometric detection of glucose as a model system for the first time. The presence of TMV nanotubes or CPs on the sensor surface allows binding of a high amount of precisely positioned enzymes without substantial loss of their activity, and may also ensure accessibility of their active centers for analyte molecules. Specific and efficient immobilization of streptavidin-conjugated GOD ([SA]-GOD) complexes on biotinylated TMV nanotubes or CPs was achieved via bioaffinity binding. These layouts were tested in parallel with glucose sensors with adsorptively immobilized [SA]-GOD, as well as [SA]-GOD crosslinked with glutardialdehyde, and came out to exhibit superior sensor performance. The achieved results underline a great potential of an integration of virus/biomolecule hybrids with electronic transducers for future applications in biosensorics and biochips.
The LAPS (light-addressable potentiometric sensor) platform is one of the most attractive approaches for chemical and biological sensing with many applications ranging from pH and ion/analyte concentration measurements up to cell metabolism detection and chemical imaging. However, although it is generally accepted that LAPS measurements are spatially resolved, the light-addressability feature of LAPS devices has not been discussed in detail so far. In this work, an extended electrical equivalent-circuit model of the LAPS has been presented, which takes into account possible cross-talk effects due to the capacitive coupling of the non-illuminated region. A shunting effect of the non-illuminated area on the measured photocurrent and addressability of LAPS devices has been studied. It has been shown, that the measured photocurrent will be determined not only by the local interfacial potential in the illuminated region but also by possible interfacial potential changes in the non-illuminated region, yielding cross-talk effects. These findings were supported by the experimental investigations of a penicillin-sensitive multi-spot LAPS and a metal-insulator-semiconductor LAPS as model systems.
Towards inclusion of the freight rail system in the industrial internet of things - Wagon 4.0
(2017)
Momentan finden in vielen Branchen umfassende Veränderungen von Märkten und Wertschöpfungsketten statt, welche auch als Digitale Transformation bezeichnet werden. In diesem Zusammenhang wird das Internet der Dinge (Internet of Things, IoT) als ein wichtiger technischer Enabler der Veränderungen angesehen. Primäre Ziele des IoT sind die Steuerung physischer Gegen-stände aus der Distanz und das Erfassen von Informationen aus dem Umfeld dieser Gegenstände. Welche neuen Geschäfts-bzw. Partnermodelle entstehen durch die gemeinsame Nutzung von IoT-Daten und Big-Data-Technologien und welcher qualitative Mehrwert wird dadurch geschaffen? Als Antwort wird in diesem Beitrag ein Bewertungsrahmen zur qualitativen Wertschöpfungsanalyse von IoT vorgeschlagen. Anhand dieses Bewertungsrahmens wird ein Anwendungsfall untersucht, der in anonymisierter Form an konkrete Praxisprojekte angelehnt ist. Konkret wird ein Anwendungsfall betrachtet, der eine Abfallwirtschaft 2.0 basierend auf dem Einsatz von IoT vorschlägt. Aus den Untersuchungsergebnissen gehen beispielsweise Erkenntnisse hervor, wie Geschäftsmodelle auf Basis eines unentgeltlichen Informationsaustauschs durch IoT gestaltet werden können.
Unwirksamkeit von Allgemeinverbindlicherklärungen des Tarifvertrages über das Sozialkassenverfahren
(2017)
This paper proposes a quick and simplified method to describe masonry vaults in global seismic analyses of buildings. An equivalent macro-element constituted by a set of six trusses, two for each transverse, longitudinal and diagonal direction, is introduced. The equivalent trusses, whose stiffness is calculated by fully modeled vaults of different geometry, mechanical properties and boundary conditions, simulate the vault in both global analysis and local analysis, such as kinematic or rocking approaches. A parametric study was carried out to investigate the influence of geometrical characteristics and mechanical features on the equivalent stiffness values. The method was numerically validated by performing modal and transient analysis on a three naves-church in the elastic range. Vibration modes and displacement time-histories were compared showing satisfying agreement between the complete and the simplified models. This procedure is particularly useful in engineering practice because it allows to assess, in a simplified way, the effectiveness of strengthening interventions for reducing horizontal relative displacements between vault supports.
Nowadays, the most employed devices for recoding videos or capturing images are undoubtedly the smartphones. Our work investigates the application of source camera identification on mobile phones. We present a dataset entirely collected by mobile phones. The dataset contains both still images and videos collected by 67 different smartphones. Part of the images consists in photos of uniform backgrounds, especially collected for the computation of the RSPN. Identifying the source camera given a video is particularly challenging due to the strong video compression. The experiments reported in this paper, show the large variation in performance when testing an highly accurate technique on still images and videos.
Vorbemerkung vor § 1297
(2017)
Vorbemerkung vor § 1353
(2017)