A convenient ninhydrin assay in 96-well format for amino acid-releasing enzymes using an air-stable reagent
- An improved and convenient ninhydrin assay for aminoacylase activity measurements was developed using the commercial EZ Nin™ reagent. Alternative reagents from literature were also evaluated and compared. The addition of DMSO to the reagent enhanced the solubility of Ruhemann's purple (RP). Furthermore, we found that the use of a basic, aqueous buffer enhances stability of RP. An acidic protocol for the quantification of lysine was developed by addition of glacial acetic acid. The assay allows for parallel processing in a 96-well format with measurements microtiter plates.
Author: | Gerrit HaegerORCiD, Johannes BongaertsORCiD, Petra SiegertORCiD |
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DOI: | https://doi.org/10.1016/j.ab.2022.114819 |
ISSN: | 1096-0309 |
Parent Title (English): | Analytical Biochemistry |
Publisher: | Elsevier |
Place of publication: | Amsterdam |
Document Type: | Article |
Language: | English |
Year of Completion: | 2022 |
Date of first Publication: | 2022/07/15 |
Issue: | 624 |
Length: | Artikel 114819 |
Link: | https://doi.org/10.1016/j.ab.2022.114819 |
Zugriffsart: | weltweit |
Institutes: | FH Aachen / Fachbereich Chemie und Biotechnologie |
FH Aachen / INB - Institut für Nano- und Biotechnologien | |
open_access (DINI-Set): | open_access |
collections: | Verlag / Elsevier |
Open Access / Hybrid | |
Licence (German): | Creative Commons - Namensnennung-Nicht kommerziell-Keine Bearbeitung |