Development of a temperature‐pulse enhanced electrochemical glucose biosensor and characterization of its stability via scanning electrochemical microscopy
- Glucose oxidase (GOx) is an enzyme frequently used in glucose biosensors. As increased temperatures can enhance the performance of electrochemical sensors, we investigated the impact of temperature pulses on GOx that was drop-coated on flattened Pt microwires. The wires were heated by an alternating current. The sensitivity towards glucose and the temperature stability of GOx was investigated by amperometry. An up to 22-fold increase of sensitivity was observed. Spatially resolved enzyme activity changes were investigated via scanning electrochemical microscopy. The application of short (<100 ms) heat pulses was associated with less thermal inactivation of the immobilized GOx than long-term heating.
| Verfasserangaben: | Stefan Wert, Heiko IkenORCiD, Michael Josef SchöningORCiD, Frank-Michael Matysik |
|---|---|
| DOI: | https://doi.org/10.1002/elan.202100089 |
| ISSN: | 1521-4109 |
| Titel des übergeordneten Werkes (Englisch): | Electroanalysis |
| Verlag: | Wiley-VCH |
| Verlagsort: | Weinheim |
| Dokumentart: | Wissenschaftlicher Artikel |
| Sprache: | Englisch |
| Erscheinungsjahr: | 2021 |
| Datum der Publikation (Server): | 13.04.2021 |
| Ausgabe / Heft: | Early View |
| Link: | https://doi.org/10.1002/elan.202100089 |
| Zugriffsart: | weltweit |
| Fachbereiche und Einrichtungen: | FH Aachen / Fachbereich Medizintechnik und Technomathematik |
| FH Aachen / INB - Institut für Nano- und Biotechnologien | |
| collections: | Verlag / Wiley-VCH |
| Open Access / Hybrid | |
| Lizenz (Deutsch): |  Creative Commons - Namensnennung |
