Refine
Document Type
- Conference Proceeding (13)
- Article (6)
- Part of a Book (3)
- Doctoral Thesis (1)
Language
- English (23) (remove)
Keywords
- Cardiac myocytes (1)
- Cardiac tissue (1)
- CellDrum (1)
- Computational biomechanics (1)
- Drug simulation (1)
- ES-FEM (1)
- Electromechanical modeling (1)
- FS-FEM (1)
- Frequency adaption (1)
- Heart tissue culture (1)
- Hodgkin–Huxley models (1)
- Homogenization (1)
- Induced pluripotent stem cells (1)
- Inotropic compounds (1)
- Ion channels (1)
- Muscle fibers (1)
- Passive stretching (1)
- Pelvic floor dysfunction (1)
- Pelvic muscle (1)
- Pharmacology (1)
Institute
- IfB - Institut für Bioengineering (23) (remove)
Background/Aims: Common systems for the quantification of cellular contraction rely on animal-based models, complex experimental setups or indirect approaches. The herein presented CellDrum technology for testing mechanical tension of cellular monolayers and thin tissue constructs has the potential to scale-up mechanical testing towards medium-throughput analyses. Using hiPS-Cardiac Myocytes (hiPS-CMs) it represents a new perspective of drug testing and brings us closer to personalized drug medication. Methods: In the present study, monolayers of self-beating hiPS-CMs were grown on ultra-thin circular silicone membranes and deflect under the weight of the culture medium. Rhythmic contractions of the hiPS-CMs induced variations of the membrane deflection. The recorded contraction-relaxation-cycles were analyzed with respect to their amplitudes, durations, time integrals and frequencies. Besides unstimulated force and tensile stress, we investigated the effects of agonists and antagonists acting on Ca²⁺ channels (S-Bay K8644/verapamil) and Na⁺ channels (veratridine/lidocaine). Results: The measured data and simulations for pharmacologically unstimulated contraction resembled findings in native human heart tissue, while the pharmacological dose-response curves were highly accurate and consistent with reference data. Conclusion: We conclude that the combination of the CellDrum with hiPS-CMs offers a fast, facile and precise system for pharmacological, toxicological studies and offers new preclinical basic research potential.