Refine
Year of publication
Document Type
- Article (100)
- Conference Proceeding (29)
- Part of a Book (12)
- Book (4)
- Other (3)
Language
- English (148) (remove)
Has Fulltext
- no (148) (remove)
Keywords
- Biocomposites (2)
- Limit analysis (2)
- Natural fibres (2)
- Polymer-matrix composites (2)
- Shakedown analysis (2)
- Stress concentrations (2)
- damage (2)
- Anastomotic leakage (1)
- Autolysis (1)
- Bladder (1)
- Bone sawing (1)
- Cardiac myocytes (1)
- Cardiac tissue (1)
- CellDrum (1)
- Chance constrained programming (1)
- Computational biomechanics (1)
- Constitutive model (1)
- Damage mechanics theory (1)
- Decomposition (1)
- Discontinuous fractures (1)
Institute
- Fachbereich Medizintechnik und Technomathematik (148) (remove)
Background/Aims: Common systems for the quantification of cellular contraction rely on animal-based models, complex experimental setups or indirect approaches. The herein presented CellDrum technology for testing mechanical tension of cellular monolayers and thin tissue constructs has the potential to scale-up mechanical testing towards medium-throughput analyses. Using hiPS-Cardiac Myocytes (hiPS-CMs) it represents a new perspective of drug testing and brings us closer to personalized drug medication. Methods: In the present study, monolayers of self-beating hiPS-CMs were grown on ultra-thin circular silicone membranes and deflect under the weight of the culture medium. Rhythmic contractions of the hiPS-CMs induced variations of the membrane deflection. The recorded contraction-relaxation-cycles were analyzed with respect to their amplitudes, durations, time integrals and frequencies. Besides unstimulated force and tensile stress, we investigated the effects of agonists and antagonists acting on Ca²⁺ channels (S-Bay K8644/verapamil) and Na⁺ channels (veratridine/lidocaine). Results: The measured data and simulations for pharmacologically unstimulated contraction resembled findings in native human heart tissue, while the pharmacological dose-response curves were highly accurate and consistent with reference data. Conclusion: We conclude that the combination of the CellDrum with hiPS-CMs offers a fast, facile and precise system for pharmacological, toxicological studies and offers new preclinical basic research potential.