Refine
Year of publication
Institute
- Fachbereich Chemie und Biotechnologie (918) (remove)
Language
- English (553)
- German (362)
- Multiple languages (2)
- Spanish (1)
Document Type
- Article (603)
- Patent (119)
- Book (66)
- Conference: Meeting Abstract (56)
- Conference Proceeding (35)
- Part of a Book (21)
- Report (6)
- Doctoral Thesis (4)
- Bachelor Thesis (3)
- Conference Poster (1)
Keywords
- Heparin (3)
- Bacillaceae (2)
- Biorefinery (2)
- Biotechnological application (2)
- Butanol (2)
- Chemometrics (2)
- IR spectroscopy (2)
- NMR spectroscopy (2)
- Principal component analysis (2)
- Standardization (2)
- Subtilases (2)
- Subtilisin (2)
- bubble column (2)
- methanation (2)
- plug flow reactor (2)
- qNMR (2)
- renewable resources (2)
- (Poly)saccharides (1)
- (R)- or (S)- gamma-valerolactone (1)
- 4-hydroxy valeric acid (1)
- ABE (1)
- Acid crash (1)
- Adsorbentien (1)
- Adsorption (1)
- Algal Turf Scrubber (1)
- Algal–bacterial bioflm (1)
- Alginate beads (1)
- Alkalihalobacillus okhensis (1)
- Aloe vera (1)
- Analytics (1)
- Authenticity (1)
- Bioeconomy (1)
- Bioethanol (1)
- Biofuel (1)
- Biogas (1)
- Biomass (1)
- Biorefinery definitions (1)
- Bladder (1)
- Bragg peak (1)
- Broad pH spectrum (1)
- C. acetobutylicum (1)
- CRISPR/Cas9 (1)
- Carrier solvents (1)
- Chimeric liver-humanized mice (1)
- Chiralidon-R (1)
- Chiralidon-S (1)
- Chondroitin sulfate (1)
- Circular bioeconomy (1)
- Clostridium acetobutylicum (1)
- Crude heparin (1)
- Cyclotron production (1)
- Decentral (1)
- Dehydrogenase (1)
- Detergent protease (1)
- Deuterated solvents (1)
- Deuterium NMR (1)
- Diaphorase (1)
- Dietary supplements (1)
- Drug distribution (1)
- Drug metabolism (1)
- Electronic cigarettes (1)
- Enzymatic biosensor (1)
- Enzymatischer Ligninabbau (1)
- Extracellular enzymes (1)
- Free-base nicotine (1)
- Ga-68 (1)
- Genetischer Algorithmus (1)
- Glucosamine (1)
- Growth modelling (1)
- Halotolerant protease (1)
- High-field NMR (1)
- Hypersecretion (1)
- IR (1)
- Inorganic ions (1)
- Introduction (1)
- Ions (1)
- Knockout mice (1)
- Levulinic acid (1)
- Lignocellulose (1)
- Lignocellulose feedstook (1)
- Lignocellulose-Bioraffinerie (1)
- Linear discriminant analysis (1)
- Low field NMR (1)
- Magnetische Adsorbermaterialien (1)
- Manufacturer (1)
- Marker-free mutagenesis (1)
- Mechanical (1)
- Mechanical simulation (1)
- Medical radionuclide production (1)
- Metabolic shift (1)
- Metal contaminants (1)
- Methane (1)
- Microfluidic solvent extraction (1)
- Minor chemistry (1)
- Molecular modelling (1)
- Molecular weight determination (1)
- Molkenprotein (1)
- Molkeproteine (1)
- NMR (1)
- On-site (1)
- Organic acids (1)
- P2G (1)
- PLS-regression (1)
- Physical chemistry (1)
- Physical chemistry basics (1)
- Physical chemistry starters (1)
- Physikalische Chemie (1)
- Polysaccharides (1)
- Pre-culture (1)
- Pre-treatment (1)
- Process schemes (1)
- Prozessintegration (1)
- Quality control (1)
- Quantum chemistry (1)
- Reconstruction (1)
- Renewable resources (1)
- Simultaneous determination (1)
- Soft independent modeling of class analogy (1)
- Spectroscopy (1)
- Stenotrophomonas maltophilia (1)
- Streptomyces griseus (1)
- Streptomyces lividans (1)
- Thermodynamics as minor (1)
- Toxicology (1)
- USP (1)
- Uracil-phosphoribosyltransferase (1)
- Ureter (1)
- Weak organic acids (1)
- Wärmeübertragung (1)
- acetoin (1)
- acetoin reductase (1)
- actuator-sensor system (1)
- alcoholic beverages (1)
- aspergillus (1)
- bacterial cellulose (1)
- bi-enzyme biosensor (1)
- bio-based economy (1)
- bio-methane (1)
- bioavailability (1)
- biodegradable polymers (1)
- biological dosimeter (1)
- biological hydrogen (1)
- biomethane (1)
- biosensors (1)
- borehole disposal (1)
- capacitive field-effect sensor (1)
- capacitive field-effect sensors (1)
- coculture (1)
- dark fermentation (1)
- deficit irrigation (1)
- detergent protease (1)
- disposal facility (1)
- drug metabolising enzymes (1)
- drug–drug interactions (1)
- elastomers (1)
- ensiling (1)
- enzyme kinetics (1)
- enzyme-logic gate (1)
- exopolysaccharides (1)
- fatty acid (1)
- fermentation (1)
- filamentous fungi (1)
- genome engineering (1)
- geological disposal (1)
- glycine (1)
- halotolerant protease (1)
- high-alkaline subtilisin (1)
- human metabolites (1)
- hydrogel (1)
- hydrogels (1)
- hydrolysis (1)
- lactic acid (1)
- lactobacillus (1)
- light-addressable electrode (1)
- light-addressable potentiometric sensor (1)
- mechanical properties (1)
- metathesis (1)
- microfluidics (1)
- micronutrients (1)
- neutrons (1)
- nuclear waste (1)
- onion (1)
- optical fibers (1)
- organic waste (1)
- oxidative stable protease (1)
- penicillinase (1)
- perennial ryegrass (1)
- polyamide (1)
- polyaspartic acid (1)
- polyester (1)
- power-to-gas (1)
- prebiotic (1)
- pretreatment (1)
- proton therapy (1)
- protons (1)
- pullulan (1)
- recombinant expression (1)
- relative dosimetry (1)
- retention time (1)
- rubber (1)
- superabsorbent polymers (1)
- supramolecular structures (1)
- swelling properties (1)
- theory and modeling (1)
- tobacco mosaic virus (TMV) (1)
- transporters (1)
- urease (1)
- water economy (1)
- yield (1)
- α-aminoacylase (1)
- β-Lactame (1)
- ε-lysine acylase (1)
The gene encoding a putative (R,R)-butane-2,3-diol dehydrogenase (bdhA) from Bacillus clausii DSM 8716T was isolated, sequenced and expressed in Escherichia coli. The amino acid sequence of the encoded protein is only distantly related to previously studied enzymes (identity 33–43%) and exhibited some uncharted peculiarities. An N-terminally StrepII-tagged enzyme variant was purified and initially characterized. The isolated enzyme catalyzed the (R)-specific oxidation of (R,R)- and meso-butane-2,3-diol to (R)- and (S)-acetoin with specific activities of 12 U/mg and 23 U/mg, respectively. Likewise, racemic acetoin was reduced with a specific activity of up to 115 U/mg yielding a mixture of (R,R)- and meso-butane-2,3-diol, while the enzyme reduced butane-2,3-dione (Vmax 74 U/mg) solely to (R,R)-butane-2,3-diol via (R)-acetoin. For these reactions only activity with the co-substrates NADH/NAD+ was observed. The enzyme accepted a selection of vicinal diketones, α-hydroxy ketones and vicinal diols as alternative substrates. Although the physiological function of the enzyme in B. clausii remains elusive, the data presented herein clearly demonstrates that the encoded enzyme is a genuine (R,R)-butane-2,3-diol dehydrogenase with potential for applications in biocatalysis and sensor development.
[{ReN(PMe2Ph)3}{ReO3N}]2 – Structural Evidence for the Nitridotrioxorhenate(VII) Anion, [ReO3N]2−
(2005)
Mice that have been genetically humanized for proteins involved in drug metabolism and toxicity and mice engrafted with human hepatocytes are emerging and promising in vivo models for an improved prediction of the pharmacokinetic, drug–drug interaction and safety characteristics of compounds in humans. The specific advantages and disadvantages of these models should be carefully considered when using them for studies in drug discovery and development. Here, an overview on the corresponding genetically humanized and chimeric liver humanized mouse models described to date is provided and illustrated with examples of their utility in drug metabolism and toxicity studies. We compare the strength and weaknesses of the two different approaches, give guidance for the selection of the appropriate model for various applications and discuss future trends and perspectives.