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Erscheinungsjahr
- 2021 (15) (entfernen)
Institut
- INB - Institut für Nano- und Biotechnologien (15) (entfernen)
Sprache
- Englisch (15) (entfernen)
Dokumenttyp
Schlagworte
- capacitive field-effect sensor (2)
- Capacitive field-effect sensor (1)
- Label-free detection (1)
- Plant virus (1)
- TMV adsorption (1)
- Ta₂O₅ gate (1)
- Tobacco mosaic virus (TMV) (1)
- Zeta potential (1)
- acetoin (1)
- acetoin reductase (1)
- actuator-sensor system (1)
- alcoholic beverages (1)
- bioburdens (1)
- biosensors (1)
- capacitive EIS sensor (1)
- capacitive electrolyte–insulator–semiconductor sensors (1)
- capacitive field-effect sensors (1)
- colorization (1)
- control gate (1)
- detection of charged macromolecules (1)
- enzymatic biosensor (1)
- enzyme kinetics (1)
- equivalent circuit (1)
- field-effect sensor (1)
- frequency mixing magnetic detection (1)
- gold nanoparticles (1)
- graphene oxide (1)
- layer-by-layer technique (1)
- light-addressable electrode (1)
- light-addressable potentiometric sensor (1)
- magnetic nanoparticles (1)
- microfluidics (1)
- multianalyte detection (1)
- multiplex detection (1)
- nanomaterials (1)
- on-chip integrated addressable EISCAP sensors (1)
- penicillin (1)
- penicillinase (1)
- photoelectrochemistry (1)
- plant virus detection (1)
- polyaniline (1)
- polystyrene sulfonate (1)
- sterility tests (1)
- sterilization efficacy (1)
- sterilization methods (1)
- titanium dioxide photoanode (1)
- tobacco mosaic virus (TMV) (1)
- validation methods (1)
Photoelectrochemical (PEC) biosensors are a rather novel type of biosensors thatutilizelighttoprovideinformationaboutthecompositionofananalyte,enablinglight-controlled multi-analyte measurements. For enzymatic PEC biosensors,amperometric detection principles are already known in the literature. In con-trast, there is only a little information on H+-ion sensitive PEC biosensors. Inthis work, we demonstrate the detection of H+ions emerged by H+-generatingenzymes, exemplarily demonstrated with penicillinase as a model enzyme on atitanium dioxide photoanode. First, we describe the pH sensitivity of the sensorand study possible photoelectrocatalytic reactions with penicillin. Second, weshow the enzymatic PEC detection of penicillin.
Biologically sensitive field-effect devices (BioFEDs) advantageously combine the electronic field-effect functionality with the (bio)chemical receptor’s recognition ability for (bio)chemical sensing. In this review, basic and widely applied device concepts of silicon-based BioFEDs (ion-sensitive field-effect transistor, silicon nanowire transistor, electrolyte-insulator-semiconductor capacitor, light-addressable potentiometric sensor) are presented and recent progress (from 2019 to early 2021) is discussed. One of the main advantages of BioFEDs is the label-free sensing principle enabling to detect a large variety of biomolecules and bioparticles by their intrinsic charge. The review encompasses applications of BioFEDs for the label-free electrical detection of clinically relevant protein biomarkers, deoxyribonucleic acid molecules and viruses, enzyme-substrate reactions as well as recording of the cell acidification rate (as an indicator of cellular metabolism) and the extracellular potential.
The treatment method to deactivate viable microorganisms from objects or products is termed sterilization. There are multiple forms of sterilization, each intended to be applied for a specific target, which depends on—but not limited to—the thermal, physical, and chemical stability of that target. Herein, an overview on the currently used sterilization processes in the global market is provided. Different sterilization techniques are grouped under a category that describes the method of treatment: radiation (gamma, electron beam, X-ray, and ultraviolet), thermal (dry and moist heat), and chemical (ethylene oxide, ozone, chlorine dioxide, and hydrogen peroxide). For each sterilization process, the typical process parameters as defined by regulations and the mode of antimicrobial activity are summarized. Finally, the recommended microorganisms that are used as biological indicators to validate sterilization processes in accordance with the rules that are established by various regulatory agencies are summarized.