Refine
Year of publication
- 2017 (154) (remove)
Institute
- Fachbereich Medizintechnik und Technomathematik (61)
- IfB - Institut für Bioengineering (32)
- INB - Institut für Nano- und Biotechnologien (26)
- Fachbereich Elektrotechnik und Informationstechnik (25)
- Fachbereich Luft- und Raumfahrttechnik (22)
- Fachbereich Energietechnik (14)
- Fachbereich Chemie und Biotechnologie (12)
- Fachbereich Maschinenbau und Mechatronik (10)
- MASKOR Institut für Mobile Autonome Systeme und Kognitive Robotik (10)
- Fachbereich Bauingenieurwesen (7)
Language
- English (154) (remove)
Document Type
- Article (72)
- Conference Proceeding (66)
- Part of a Book (12)
- Other (2)
- Book (1)
- Patent (1)
Keywords
- MASCOT (2)
- 3D nonlinear finite element model (1)
- Acceptance tests (1)
- Automated Optimization (1)
- Autonomous mobile robots (1)
- Biomolecular logic gate (1)
- CNOT (1)
- Capacitive field-effect (1)
- Chemical images (1)
- Chemical sensor (1)
Is part of the Bibliography
- no (154)
Genetically humanized mice for proteins involved in drug metabolism and toxicity and mice engrafted with human hepatocytes are emerging as promising in vivo models for improved prediction of the pharmacokinetic, drug–drug interaction, and safety characteristics of compounds in humans. This is an overview on the genetically humanized and chimeric liver-humanized mouse models, which are illustrated with examples of their utility in drug metabolism and toxicity studies. The models are compared to give guidance for selection of the most appropriate model by highlighting advantages and disadvantages to be carefully considered when used for studies in drug discovery and development.
In this paper, carbon nanotubes (CNTs) were incorporated in penicillinase-phospholipid Langmuir and Langmuir–Blodgett (LB) films to enhance the enzyme catalytic properties. Adsorption of the penicillinase and CNTs at dimyristoylphosphatidic acid (DMPA) monolayers at the air–water interface was investigated by surface pressure–area isotherms, vibrational spectroscopy, and Brewster angle microscopy. The floating monolayers were transferred to solid supports through the LB technique, forming mixed DMPA-CNTs-PEN films, which were investigated by quartz crystal microbalance, vibrational spectroscopy, and atomic force microscopy. Enzyme activity was studied with UV–vis spectroscopy and the feasibility of the supramolecular device nanostructured as ultrathin films were essayed in a capacitive electrolyte–insulator–semiconductor (EIS) sensor device. The presence of CNTs in the enzyme–lipid LB film not only tuned the catalytic activity of penicillinase but also helped conserve its enzyme activity after weeks, showing increased values of activity. Viability as penicillin sensor was demonstrated with capacitance/voltage and constant capacitance measurements, exhibiting regular and distinctive output signals over all concentrations used in this work. These results may be related not only to the nanostructured system provided by the film, but also to the synergism between the compounds on the active layer, leading to a surface morphology that allowed a fast analyte diffusion because of an adequate molecular accommodation, which also preserved the penicillinase activity. This work therefore demonstrates the feasibility of employing LB films composed of lipids, CNTs, and enzymes as EIS devices for biosensing applications.