Refine
Year of publication
Document Type
- Article (122) (remove)
Has Fulltext
- yes (122) (remove)
Keywords
- Einspielen <Werkstoff> (7)
- Multimediamarkt (6)
- Rapid prototyping (5)
- FEM (4)
- Finite-Elemente-Methode (4)
- Rapid Prototyping (4)
- Blitzschutz (3)
- 3D-Printing (2)
- Acyl-amino acids (2)
- Aminoacylase (2)
- Bacillaceae (2)
- Bauingenieurwesen (2)
- Biotechnological application (2)
- CAD (2)
- CFD (2)
- Capacitive field-effect sensor (2)
- Einspielanalyse (2)
- Hochschuldidaktik (2)
- Karosseriebau (2)
- Kraftfahrzeugbau (2)
- Label-free detection (2)
- Lightning protection (2)
- Mikrosystemtechnik (2)
- Rapid Manufacturing (2)
- Shakedown analysis (2)
- Sportwagen (2)
- Stereolithographie (2)
- Studentenprojekt (2)
- Subtilases (2)
- Subtilisin (2)
- Traglastanalyse (2)
- UAV (2)
- Virtuelle Fahrzeugentwicklung (2)
- biosensors (2)
- capacitive field-effect sensors (2)
- car body construction (2)
- civil engineering (2)
- rapid manufacturing (2)
- shakedown (2)
- shakedown analysis (2)
- (Poly)saccharides (1)
- Acylation (1)
- Aeroelasticity (1)
- Air purification (1)
- Aircraft sizing (1)
- Algal Turf Scrubber (1)
- Algal–bacterial bioflm (1)
- Alkalihalobacillus okhensis (1)
- Aloe vera (1)
- Alternating plasticity (1)
- Alternative Energiequelle (1)
- Analysis (1)
- Analytischer Zulaessigkeitsnachweis (1)
- Anammox (1)
- Anforderungen (1)
- Arbeitsbelastung (1)
- Architectural design (1)
- Architektur (1)
- Arthosetherapie (1)
- Aufschlagversuch (1)
- Automotive safety approach (1)
- Autonomy (1)
- Axialbelastung (1)
- Axially cracked pipe (1)
- Bacterial cellulose (1)
- Basis Reduktion (1)
- Basis reduction (1)
- Baubetrieb (1)
- Baukosten (1)
- Bauvertrag (1)
- Bewertung von Studienleistungen (1)
- Bicharakteristikenverfahren (1)
- Bioabsorbable (1)
- Biocatalysis (1)
- Biofuel (1)
- Biogas (1)
- Bioreaktor (1)
- Biorefinery (1)
- Biosurfactants (1)
- Blade element method (1)
- Blutzellenlagerung (1)
- Bologna-Prozess (1)
- Bone quality and biomechanics (1)
- Brake set-up (1)
- Brake test (1)
- Broad pH spectrum (1)
- Butanol (1)
- Carbon sources (1)
- Cellulose nanostructure (1)
- Cement infiltration (1)
- Chaostheorie (1)
- Chaperone (1)
- Chaperone co-expression (1)
- Charging station (1)
- Chondroitin sulfate (1)
- Circular bioeconomy (1)
- Clostridium acetobutylicum (1)
- Clusterion (1)
- Coaching (1)
- Content Management (1)
- Convex optimization (1)
- Cost-effectiveness (1)
- Crashworthiness (1)
- Culture media (1)
- Deformation (1)
- Design-by-analysis (1)
- Designpraxis (1)
- Didactics (1)
- Dietary supplements (1)
- Digital Museum (1)
- Digitales Museum (1)
- Drag estimation (1)
- Druckbeanspruchung (1)
- Druckbehälter (1)
- Druckbelastung (1)
- Druckgeräte (1)
- E-Learning (1)
- ECTS (1)
- ELISA (1)
- Einbetten in das Internet der Dinge (1)
- Einspiel-Analyse (1)
- Einspiel-Kriterium (1)
- Einspielen (1)
- Elastizität (1)
- Electronic Art (1)
- Electronic vehicle (1)
- Elektronische Kunst (1)
- Energietechnische Anlage (1)
- Enterprise information systems (1)
- Environmental impact (1)
- Enzyme-linked immunosorbent assay (1)
- Epithel (1)
- European Credit Transfer System (1)
- Europäisches Leistungspunktesystem (1)
- Experiment (1)
- Extrusionsverfahren (1)
- FLM (1)
- Fault approximation (1)
- Fault detection (1)
- Fehlerstellen (1)
- Fibroblast (1)
- Finite element method (1)
- Fließgrenze (1)
- Freight rail (1)
- Full-vehicle crash test (1)
- Fused deposition modelling (1)
- Fußball (1)
- Generative Fertigungstechnik (1)
- Geriatric (1)
- German (1)
- Gesundheitswesen (1)
- Gießharzwerkzeuge (1)
- Global and local collapse (1)
- Glucosamine (1)
- Gold nanoparticles (1)
- Gonarthrose (1)
- Grenzwertberechnung (1)
- Halotolerant protease (1)
- Harnleiter (1)
- Hemoglobin structure (1)
- High-field NMR (1)
- Hip fractures (1)
- Hämoglobin (1)
- Hämoglobinstruktur (1)
- Incident analysis (1)
- Inclusion bodies (1)
- Information extraction (1)
- Innenströmung (1)
- Inverse scattering problem (1)
- Kniegelenkarthrose (1)
- Knochen (1)
- Knochenbildung (1)
- Knochenchirugie (1)
- Knochendichte (1)
- Kohlenstofffaser (1)
- Kontaktstunden (1)
- Kostenermittlung (1)
- Kostenkontrolle (1)
- Kostensteuerung (1)
- Laminat Verfahren (1)
- Laminated-Object-Manufacturing (1)
- Lasersintern (1)
- Laserstrahlsintern (1)
- Lignocellulose (1)
- Limit analysis (1)
- Lipopolysaccharide (1)
- Local path planning (1)
- Luftreiniger (1)
- MBST (1)
- MCDA (1)
- MPEG 4 (1)
- MPEG 7 (1)
- MPEG-Standard (1)
- MST (1)
- Mainstream (1)
- Materialermüdung (1)
- Medusomyces gisevi (1)
- Methane (1)
- Mikrotechnik (1)
- Minimal-Ansatz für Embedded-Systeme (1)
- Model-driven software engineering (1)
- Modellkanäle (1)
- Multicell (1)
- Multimedia-Dienst (1)
- Multiplexing (1)
- Multirotor UAS (1)
- Museum (1)
- Museumsorganisation (1)
- Netzkunst (1)
- Nitrogen removal (1)
- Obstacle avoidance (1)
- Organic acids (1)
- Organkultur (1)
- Osteoporose (1)
- Osteoporosis (1)
- Oxidationsofen (1)
- Oxidschicht (1)
- Parasitic drag (1)
- Parking (1)
- Partial nitritation (1)
- Permeability (1)
- Permeabilität (1)
- Peroxidase (1)
- Pflanzenphysiologie (1)
- Pflanzenscanner (1)
- Pflanzenstress (1)
- Photolithographie (1)
- Plant virus (1)
- Plasmacluster ion technology (1)
- Plastizität (1)
- Polylactide acid (1)
- Polysaccharides (1)
- Pressure loaded crack-face (1)
- Prevention (1)
- Progressive plastic deformation (1)
- Projektsteuerung (1)
- Propeller (1)
- Propeller elasticity (1)
- Prophylaxis (1)
- Prozessautomatisierung (1)
- Prozessverbesserung (1)
- RPA (1)
- Rapid Technologie (1)
- Ratchetting (1)
- Raumluft (1)
- Red blood cell storage (1)
- Regenerative Energieanlagen (1)
- Reservation system (1)
- Resistive temperature detector (1)
- Risikoabschätzung (1)
- Risikomanagement (1)
- Risk assessment (1)
- Risk management (1)
- Robotic Process Automation (1)
- Rohr (1)
- Rohrbruch (1)
- Schienbeinschoner (1)
- Schwammknochen (1)
- Selektionskriterien (1)
- Shakedown (1)
- Shakedown criterion (1)
- Silk fibroin (1)
- Simulation (1)
- Skeletal muscle (1)
- Small Aral Sea (1)
- Software and systems modeling (1)
- Spectroscopy (1)
- Sprachassistent (1)
- Sprachsteuerung (1)
- Stahl (1)
- Streptomyces griseus (1)
- Streptomyces lividans (1)
- Strukturanalyse (1)
- Strömungsanalyse (1)
- Temperaturabhängigkeit (1)
- Tendons (1)
- Tissue Engineering (1)
- Tobacco mosaic virus (TMV) (1)
- Traglast (1)
- Train composition (1)
- Ultrasound (1)
- Umsetzbarkeit (1)
- Unternehmernachfolge (1)
- Utilization improvement (1)
- VM (1)
- VOB (1)
- Vakuumgießen (1)
- Vernetzung (1)
- Vertebroplastie (1)
- Vertebroplasty (1)
- Vertragsbedingungen (1)
- Vibrio natriegens (1)
- Virtual Art (1)
- Virtual Exhibition (1)
- Virtual Museum (1)
- Virtuelle Ausstellung (1)
- Virtuelle Maschine (1)
- Virtuelles Museum (1)
- Viscous flow (1)
- Viskose Strömung (1)
- Viskosität (1)
- Wastewater (1)
- Werkzeuge (1)
- Werkzeugeinsätze (1)
- Wind milling (1)
- Wind tunnel experiments (1)
- Wolff's Law (1)
- Wolffsches Gesetz (1)
- Workload (1)
- Wundheilung (1)
- Zeta potential (1)
- Zug-Druck Belastung (1)
- acetoin (1)
- acetoin reductase (1)
- additive manufacturing (1)
- alcoholic beverages (1)
- alternierend Verformbarkeit (1)
- amperometric biosensors (1)
- architecture (1)
- aromatic amines (1)
- arthrosis therapy (1)
- atomic layer deposition (1)
- autonomous navigation (1)
- bicharacteristics (1)
- bio-methane (1)
- bioburdens (1)
- biocompatible (1)
- biocompatible materials (1)
- biodegradabl (1)
- biodegradable electronic devices (1)
- blended learning (1)
- bone density (1)
- bone structure (1)
- bubble column (1)
- burst pressure (1)
- burst tests (1)
- cancellous bone (1)
- capacitive electrolyte–insulator–semiconductor sensors (1)
- carbon electrodes (1)
- carbonized rice husk (1)
- detergent protease (1)
- direct method (1)
- distribution grid simulation (1)
- e-mobility (1)
- eVTOL development (1)
- eVTOL safety (1)
- ecological structure (1)
- elastic solids (1)
- encapsulation materials (1)
- enzymatic biosensor (1)
- epithelization (1)
- factory planning (1)
- fatigue analyses (1)
- fibroin (1)
- flaw (1)
- fortschreitende plastische Deformation (1)
- generative Fertigungsverfahren (1)
- glucose (1)
- gonarthrosis (1)
- graphene oxide (1)
- halotolerant protease (1)
- high-alkaline subtilisin (1)
- human dermal fibroblasts (1)
- konvexe Optimierung (1)
- large language models (1)
- large-scale inspection (1)
- layer-by-layer technique (1)
- lightning protection (1)
- limit analysis (1)
- limit load (1)
- lipopolysaccharides (1)
- load limit (1)
- manufacturing flexibility (1)
- metagenomics (1)
- methanation (1)
- micro technology (1)
- micro-technical components (1)
- microbial diversity (1)
- mikrotechnische Komponenten (1)
- mix flexibility (1)
- mobile manipulation (1)
- multimediale Lernsoftware (1)
- nanomaterials (1)
- nanostructured carbonized plant parts (1)
- nanostrukturierte carbonisierte Pflanzenteile (1)
- naphtols (1)
- oxidation furnace (1)
- oxidative stable protease (1)
- oxide coating (1)
- pH sensors (1)
- penicillin (1)
- penicillinase (1)
- phenols (1)
- photoelectrochemistry (1)
- pipes (1)
- plant scanner (1)
- plant stress (1)
- plug flow reactor (1)
- polyaniline (1)
- power-to-gas (1)
- qNMR (1)
- ratchetting (1)
- recombinant expression (1)
- reliability analysis (1)
- renewable energy (1)
- semantic role labeling (1)
- shakedown analyses (1)
- shotgun sequencing (1)
- smart-charging (1)
- speaker attribution (1)
- sterility tests (1)
- sterilization efficacy (1)
- sterilization methods (1)
- surface-orthogonal path planning (1)
- tension–torsion loading (1)
- thermal ratcheting (1)
- titanium dioxide photoanode (1)
- ultrathin gate insulators (1)
- validation methods (1)
- vessels (1)
- virtual clean room (1)
- volume flexibility (1)
- wind turbine production (1)
- wound healing (1)
- yield stress (1)
- α-aminoacylase (1)
- ε-lysine acylase (1)
Institute
- Fachbereich Medizintechnik und Technomathematik (47)
- IfB - Institut für Bioengineering (22)
- Fachbereich Maschinenbau und Mechatronik (20)
- INB - Institut für Nano- und Biotechnologien (17)
- Fachbereich Chemie und Biotechnologie (12)
- Fachbereich Energietechnik (11)
- Fachbereich Luft- und Raumfahrttechnik (9)
- Fachbereich Elektrotechnik und Informationstechnik (8)
- ECSM European Center for Sustainable Mobility (6)
- Fachbereich Bauingenieurwesen (6)
- Nowum-Energy (6)
- Fachbereich Wirtschaftswissenschaften (4)
- Fachbereich Architektur (3)
- Fachbereich Gestaltung (3)
- ZHQ - Bereich Hochschuldidaktik und Evaluation (3)
- Solar-Institut Jülich (2)
- Arbeitsstelle fuer Hochschuldidaktik und Studienberatung (1)
- FH Aachen (1)
- IBB - Institut für Baustoffe und Baukonstruktionen (1)
- MASKOR Institut für Mobile Autonome Systeme und Kognitive Robotik (1)
The growing body of political texts opens up new opportunities for rich insights into political dynamics and ideologies but also increases the workload for manual analysis. Automated speaker attribution, which detects who said what to whom in a speech event and is closely related to semantic role labeling, is an important processing step for computational text analysis. We study the potential of the large language model family Llama 2 to automate speaker attribution in German parliamentary debates from 2017-2021. We fine-tune Llama 2 with QLoRA, an efficient training strategy, and observe our approach to achieve competitive performance in the GermEval 2023 Shared Task On Speaker Attribution in German News Articles and Parliamentary Debates. Our results shed light on the capabilities of large language models in automating speaker attribution, revealing a promising avenue for computational analysis of political discourse and the development of semantic role labeling systems.
N-Acyl-amino acids can act as mild biobased surfactants, which are used, e.g., in baby shampoos. However, their chemical synthesis needs acyl chlorides and does not meet sustainability criteria. Thus, the identification of biocatalysts to develop greener synthesis routes is desirable. We describe a novel aminoacylase from Paraburkholderia monticola DSM 100849 (PmAcy) which was identified, cloned, and evaluated for its N-acyl-amino acid synthesis potential. Soluble protein was obtained by expression in lactose autoinduction medium and co-expression of molecular chaperones GroEL/S. Strep-tag affinity purification enriched the enzyme 16-fold and yielded 15 mg pure enzyme from 100 mL of culture. Biochemical characterization revealed that PmAcy possesses beneficial traits for industrial application like high temperature and pH-stability. A heat activation of PmAcy was observed upon incubation at temperatures up to 80 °C. Hydrolytic activity of PmAcy was detected with several N-acyl-amino acids as substrates and exhibited the highest conversion rate of 773 U/mg with N-lauroyl-L-alanine at 75 °C. The enzyme preferred long-chain acyl-amino-acids and displayed hardly any activity with acetyl-amino acids. PmAcy was also capable of N-acyl-amino acid synthesis with good conversion rates. The best synthesis results were obtained with the cationic L-amino acids L-arginine and L-lysine as well as with L-leucine and L-phenylalanine. Exemplarily, L-phenylalanine was acylated with fatty acids of chain lengths from C8 to C18 with conversion rates of up to 75%. N-lauroyl-L-phenylalanine was purified by precipitation, and the structure of the reaction product was verified by LC–MS and NMR.