Refine
Year of publication
Institute
- Fachbereich Medizintechnik und Technomathematik (1699)
- Fachbereich Elektrotechnik und Informationstechnik (722)
- IfB - Institut für Bioengineering (627)
- Fachbereich Energietechnik (590)
- INB - Institut für Nano- und Biotechnologien (557)
- Fachbereich Chemie und Biotechnologie (555)
- Fachbereich Luft- und Raumfahrttechnik (500)
- Fachbereich Maschinenbau und Mechatronik (289)
- Fachbereich Wirtschaftswissenschaften (224)
- Solar-Institut Jülich (165)
Language
- English (4961) (remove)
Document Type
- Article (3277)
- Conference Proceeding (1197)
- Part of a Book (197)
- Book (146)
- Conference: Meeting Abstract (34)
- Doctoral Thesis (32)
- Patent (25)
- Other (10)
- Report (10)
- Conference Poster (6)
Keywords
- Biosensor (25)
- Finite-Elemente-Methode (12)
- Einspielen <Werkstoff> (10)
- CAD (8)
- civil engineering (8)
- Bauingenieurwesen (7)
- Blitzschutz (6)
- FEM (6)
- Gamification (6)
- Limit analysis (6)
The transgeneticist's toolbox: novel methods for the targeted modification of eukaryotic genomes
(2000)
Investigation of TRPV1 loss-of-function phenotypes in transgenic shRNA expressing and knockout mice
(2008)
The contribution of altered post-transcriptional gene silencing to the development of insulin resistance and type 2 diabetes mellitus so far remains elusive. Here, we demonstrate that expression of microRNA (miR)-143 and 145 is upregulated in the liver of genetic and dietary mouse models of obesity. Induced transgenic overexpression of miR-143, but not miR-145, impairs insulin-stimulated AKT activation and glucose homeostasis. Conversely, mice deficient for the miR-143–145 cluster are protected from the development of obesity-associated insulin resistance. Quantitative-mass-spectrometry-based analysis of hepatic protein expression in miR-143-overexpressing mice revealed miR-143-dependent downregulation of oxysterol-binding-protein-related protein (ORP) 8. Reduced ORP8 expression in cultured liver cells impairs the ability of insulin to induce AKT activation, revealing an ORP8-dependent mechanism of AKT regulation. Our experiments provide direct evidence that dysregulated post-transcriptional gene silencing contributes to the development of obesity-induced insulin resistance, and characterize the miR-143–ORP8 pathway as a potential target for the treatment of obesity-associated diabetes.
Meiotic functions of RAD18
(2011)
Obesity-induced overexpression of miR-802 impairs glucose metabolism through silencing of Hnf1b
(2013)