Refine
Year of publication
- 2023 (31) (remove)
Document Type
- Article (16)
- Part of a Book (9)
- Conference Proceeding (4)
- Habilitation (1)
- Preprint (1)
Has Fulltext
- no (31) (remove)
Keywords
- Natural language processing (3)
- Information extraction (2)
- ultrasound (2)
- Active learning (1)
- Agent-based simulation (1)
- Asymptotic relative efficiency (1)
- Bacillus atrophaeus spores (1)
- Clustering (1)
- Competitiveness (1)
- Conductive Boundary Condition (1)
- Cramér-von-Mises test (1)
- Cross border adjustment mechanism (1)
- DPA (dipicolinic acid) (1)
- Deep learning (1)
- E-Mobility (1)
- Endothelial dysfunction (1)
- Energy market design (1)
- Energy-intensive industry (1)
- Floor prices (1)
- Incomplete data (1)
- Inverse Scattering (1)
- LbL films (1)
- Long COVID (1)
- Marginal homogeneity (1)
- Market modeling (1)
- Mobility transition (1)
- Multi-criteria decision analysis (1)
- Natural language understanding (1)
- Paired sample (1)
- Post-COVID-19 syndrome (1)
- Preference assessment (1)
- Profile extraction (1)
- Query learning (1)
- Raman spectroscopy (1)
- Regionalization (1)
- Relation classification (1)
- Reproducible research (1)
- Sn₃O₄ (1)
- Steel industry (1)
- Text mining (1)
- Transmission Eigenvalues (1)
- Trustworthy artificial intelligence (1)
- Volumes of confidence regions (1)
- allocation (1)
- biomechanics (1)
- biosensor (1)
- central symmetry test (1)
- conditional excess distribution (1)
- conditional expectation principle (1)
- confidence interval (1)
- connective tissue (1)
- covariance principle (1)
- electromyography (1)
- enzyme cascade (1)
- exchangeability test (1)
- field-effect sensor (1)
- forecast (1)
- glucose oxidase (GOx) (1)
- goodness-of-fit test (1)
- heavy metals (1)
- horseradish peroxidase (HRP) (1)
- independence test (1)
- locomotion (1)
- nanobelts (1)
- not identically distributed (1)
- optical sensor setup (1)
- optical trapping (1)
- overload (1)
- physiology (1)
- portfolio risk (1)
- random effects (1)
- retinal microvasculature (1)
- sterilization (1)
- stretch-shortening cycle (1)
- tobacco mosaic virus (TMV) (1)
- turnip vein clearing virus (TVCV) (1)
Institute
- Fachbereich Medizintechnik und Technomathematik (31) (remove)
Immunosorbent turnip vein clearing virus (TVCV) particles displaying the IgG-binding domains D and E of Staphylococcus aureus protein A (PA) on every coat protein (CP) subunit (TVCVPA) were purified from plants via optimized and new protocols. The latter used polyethylene glycol (PEG) raw precipitates, from which virions were selectively re-solubilized in reverse PEG concentration gradients. This procedure improved the integrity of both TVCVPA and the wild-type subgroup 3 tobamovirus. TVCVPA could be loaded with more than 500 IgGs per virion, which mediated the immunocapture of fluorescent dyes, GFP, and active enzymes. Bi-enzyme ensembles of cooperating glucose oxidase and horseradish peroxidase were tethered together on the TVCVPA carriers via a single antibody type, with one enzyme conjugated chemically to its Fc region, and the other one bound as a target, yielding synthetic multi-enzyme complexes. In microtiter plates, the TVCVPA-displayed sugar-sensing system possessed a considerably increased reusability upon repeated testing, compared to the IgG-bound enzyme pair in the absence of the virus. A high coverage of the viral adapters was also achieved on Ta2O5 sensor chip surfaces coated with a polyelectrolyte interlayer, as a prerequisite for durable TVCVPA-assisted electrochemical biosensing via modularly IgG-assembled sensor enzymes.