Refine
Year of publication
Document Type
- Article (91) (remove)
Keywords
- CellDrum (3)
- Lipopolysaccharide (2)
- Air purification (1)
- Bioreaktor (1)
- Blutzellenlagerung (1)
- Cardiac myocytes (1)
- Cell permeability (1)
- Cellular force (1)
- Circular Dichroism (1)
- Clusterion (1)
- Contractile tension (1)
- Endothelial cells (1)
- Epithel (1)
- Fibroblast (1)
- Harnleiter (1)
- Heart tissue culture (1)
- Hemoglobin structure (1)
- Hämoglobin (1)
- Hämoglobinstruktur (1)
- Induced pluripotent stem cells (1)
Background/Aims: Common systems for the quantification of cellular contraction rely on animal-based models, complex experimental setups or indirect approaches. The herein presented CellDrum technology for testing mechanical tension of cellular monolayers and thin tissue constructs has the potential to scale-up mechanical testing towards medium-throughput analyses. Using hiPS-Cardiac Myocytes (hiPS-CMs) it represents a new perspective of drug testing and brings us closer to personalized drug medication. Methods: In the present study, monolayers of self-beating hiPS-CMs were grown on ultra-thin circular silicone membranes and deflect under the weight of the culture medium. Rhythmic contractions of the hiPS-CMs induced variations of the membrane deflection. The recorded contraction-relaxation-cycles were analyzed with respect to their amplitudes, durations, time integrals and frequencies. Besides unstimulated force and tensile stress, we investigated the effects of agonists and antagonists acting on Ca²⁺ channels (S-Bay K8644/verapamil) and Na⁺ channels (veratridine/lidocaine). Results: The measured data and simulations for pharmacologically unstimulated contraction resembled findings in native human heart tissue, while the pharmacological dose-response curves were highly accurate and consistent with reference data. Conclusion: We conclude that the combination of the CellDrum with hiPS-CMs offers a fast, facile and precise system for pharmacological, toxicological studies and offers new preclinical basic research potential.