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A light-addressable potentiometric sensor (LAPS) is a field-effect-based potentiometric sensor with an electrolyte/insulator/semiconductor (EIS) structure, which is able to monitor analyte concentrations of (bio-)chemical species in aqueous solutions in a spatially resolved way. Therefore, it is also an appropriate tool to record 2D-chemical images of concentration variations on the sensor surface. In the present work, two differential, LAPS-based measurement principles are introduced to determine the metabolic activity of Escherichia coli (E. coli) K12 and Chinese hamster ovary (CHO) cells as test microorganisms. Hereby, we focus on i) the determination of the extracellular acidification rate (ΔpH/min) after adding glucose solutions to the cell suspensions; and ii) recording the amplitude increase of the photocurrent (Iph) related to the produced acids from E. coli K12 bacteria and CHO cells on the sensor surface by 2D-chemical imaging. For this purpose, 3D-printed multi-chamber structures were developed and mounted on the planar sensor-chip surface to define four independent compartments, enabling differential measurements with varying cell concentrations. The differential concept allows eliminating unwanted drift effects and, with the four-chamber structures, measurements on the different cell concentrations were performed simultaneously, thus reducing also the overall measuring time.
Dimensionen 1-2017 : Magazin der FH Aachen University of Applied Sciences - Struktur und Freiheit
(2017)
Inhalt Dimensionen 01-2017:
6 Struktur und Freiheit : Wie die Digitalisierung die Lehre beeinflusst
12 Wie wir in Zukunft konsumieren : Vom intelligenten Kühlschrank bis zur selbst gedruckten Brille
16 Baby unter Beobachtung : Forscher der FH decken massive Sicherheitsmängel bei Babymonitoringsystem auf
18 "In unserem Auge ist viel los" : Anissa Frank hat sich in ihrer Bachelorarbeit mit dem Phänomenen des kreisrunden Makulaloches beschäftigt
20 #totallove : Was kommt nach dem "Hotel Total"?
24 Das graue Wunder : Dirk Thal, Leon Bockstegers und Erick Regehr sind überzeugt: "Beton kann mehr"
26 Auftrieb : Zwei FH-Innovationen landen beim Luftfahrtkonzern Airbus
30 Ich möchte die Kunst demokratisieren : David Gerards bietet Künstlern mit dem "Poebel" eine Plattform
32 Zwischen Orient und Okzident : Unterwegs in Marokko
38 Einfach genau hinhören und horchen : Interview mit Prof. Dr. Hans-Joachim Blome zum Thema Gravitationswellen
42 Die Entdeckung eines Architekten : Auf den Spuren des Architekten Leonardo da Vinci
46 Über den Dächern von Chicago
48 Eine Prothese aus dem 3D-Drucker : Studierende engagieren sich für ein Projekt, das vielen Menschen in Krisenregionen helfen kann
50 Ist der Tod das Ende unserer Persönlichkeit? Prof. Dr. Walter van Laack ist Experte auf dem Gebiet Nahtoderfahrungen
52 Von Aachen in die ganze Welt : Die Summer Schools sind der Exportschlager der FH Aachen
59 Ein Stipendium zum Geburtstag
60 Keine Chance für Shimmy
58 Kopfnuss
61 Neues Buch über Prof. Rudold Schwarz
62 Silber beim PR-Bild-Award
63 Impressum
Blackout : Wenn bei uns allen das Licht ausgeht
Inhaltsverzeichnis:
06 Wenn im Elfenbeinturm das Licht ausgeht: Zur gesellschaftlichen Verantwortung der Wissenschaft
12 Mekka der Solarforschung: Das Solar-Institut Jülich feiert Jubiläum- und blick nach vorn
16 Hinter den Steinen: FH Aachen und die Stiftung Smart Building entwickeln gemeinsam den neuen Studiengang Smart Building Engineering
18 Die Biogaspioniere: Das Institut NOWUM-Energy sucht neue Perspektiven für die Energieerzeugung
22 Elektrisierend: Studentin entwirft Tapuya-Bühne für Electrisize Festival
24 keep calm and don´t panic
26 Der Markt als Experimentierfeld: Die Wirtschaft wandelt sich - die FH mischt in der Gründerszene mit
30 Mit einem Studium in ein neues Leben: Betreuungskonzept für Geflüchtete an der FH Aachen
32 Spuren im Staub
34 Auf Archimedes´ Spuren: Studierende stechen mit Betonkanus in See
36 "Ich investiere in Menschen": Interview mit Alexander Wilden, Inhaber der schwartz Gruppe mit Hauptsitz in Simmerath
40 Wie im richtigen Leben: 10 Jahre pro8: Rückblick und Ausblick
42 Zwei Kreuze für die Christuskirche: Entwürfe von Architekturstudienden werden umgesetzt
44 Eine gute Ausbildung ist prkatisch: Florian Kessel macht grenzüberschreitende Karriere
46 Er will das Feuer weitergeben: Holzbaupionier Prof. Hermann Blumer lehrt an der FH
50 Aufwind für innovative Lehre: Fünf Projekte im Rahmen des SQSL-Programms ausgezeichnet
51 Der einsame Turm: Kopfnuss
52 Vom Winde verweht: Benedikt Lösch kommt bei der Segelweltmeisterschaft auf Rang 28
54 Wüstensand und fliegende Roboter: Team des MASKOR-Instituts erreicht Finalrunde beim MBZIRC-Wettbewerb
56 Die Rattan-Revolution: Das Start-up "out for space" erfindet den Werkstoff neu
Engineers and therefore engineering education are challenged by the increasing complexity of questions to be answered globally. The education of future engineers therefore has to answer with curriculums that build up relevant skills. This chapter will give an example how to bring engineering and social responsibility successful together to build engineers of tomorrow. Through the integration of gender and diversity perspectives, engineering research and teaching is expanded with new perspectives and contents providing an important potential for innovation. Aiming on the enhancement of engineering education with distinctive competencies beyond technical expertise, the teaching approach introduced in the chapter represents key factors to ensure that coming generations of engineers will be able to meet the requirements and challenges a changing globalized world holds for them. The chapter will describe how this approach successfully has been implemented in the curriculum in engineering of a leading technical university in Germany.
Contemporary research appreciates a diverse workforce as a potential source of innovation. Researchers explore the fine details of why diversity management is central for generating innovations in heterogeneous research groups and how it could be effectively implemented into organizations. Complex research associations that discuss topics with a high impact on society increasingly address the necessity of establishing a diverse workforce to confront the challenges of tomorrow. Characterized by complex management structures as well as hierarchies, research associations have not been a subject of investigation until now. For this reason, the presented research project aims to develop a diversity and innovation management strategy with the ultimate goal of inducing change in the corporate culture. The proposed approach consisted of six phases; the first two phases investigated the status quo of diversity in the existing organizational structures of member institutes and the variety of particular working cultures within the research association. The third and the fourth phases utilized qualitative and quantitative studies. The third phase focused on the connection of management level to diversity and innovation, and the need for diversity and innovation management, and tailor-made methods of implementing them. The first three phases have been accomplished successfully; preliminary results are already available. The fourth phase will mainly focus on exploring the mind-set of the employees. The fifth phase will consolidate the findings in the first four phases into an implementable strategy. The final phase will address the implementation of this strategy into the organization. Phases 4 to 6 have not yet been undertaken
An array of four independently wired indium tin oxide (ITO) electrodes was used for electrochemically stimulated DNA release and activation of DNA-based Identity, AND and XOR logic gates. Single-stranded DNA molecules were loaded on the mixed poly(N,N-dimethylaminoethyl methacrylate) (PDMAEMA)/poly(methacrylic acid) (PMAA) brush covalently attached to the ITO electrodes. The DNA deposition was performed at pH 5.0 when the polymer brush is positively charged due to protonation of tertiary amino groups in PDMAEMA, thus resulting in electrostatic attraction of the negatively charged DNA. By applying electrolysis at −1.0 V(vs. Ag/AgCl reference) electrochemical oxygen reduction resulted in the consumption of hydrogen ions and local pH increase near the electrode surface. The process resulted in recharging the polymer brush to the negative state due to dissociation of carboxylic groups of PMAA, thus repulsing the negatively charged DNA and releasing it from the electrode surface. The DNA release was performed in various combinations from different electrodes in the array assembly. The released DNA operated as input signals for activation of the Boolean logic gates. The developed system represents a step forward in DNA computing, combining for the first time DNA chemical processes with electronic input signals.
The telecommunications industry is currently going through a major transformation. In this context, the enhanced Telecom Operations Map (eTOM) is a domain-specific process reference model that is offered by the industry organization TM Forum. In practice, eTOM is well accepted and confirmed as de facto standard. It provides process definitions and process flows on different levels of detail. This article discusses the reference modeling of eTOM, i.e., the design, the resulting artifact, and its evaluation based on three project cases. The application of eTOM in three projects illustrates the design approach and concrete models on strategic and operational levels. The article follows the Design Science Research (DSR) paradigm. It contributes with concrete design artifacts to the transformational needs of the telecommunications industry and offers lessons-learned from a general DSR perspective.