Open Access

In common with most infrastructure systems, sewers are often inspected visually. Currently, the results from these inspections inform decisions for significant investments regarding sewer rehabilitation or replacement. In practice, the quality of the data and its analysis are not questioned although psychological research indicates that, as a consequence of the use of subjective analysis of the collected images, errors are inevitable. This article assesses the quality of the analysis of visual sewer inspection data by analysing data reproducibility; three types of capabilities to subjectively assess data are distinguished: the recognition of defects, the description of defects according to a prescribed coding system and the interpretation of sewer inspection reports. The introduced uncertainty is studied using three types of data: inspector examination results of sewer inspection courses, data gathered in day-to-day practice, and the results of repetitive interpretation of the inspection results. After a thorough analysis of the data it can be concluded that for all cases visual sewer inspection data proved poorly reproducible. For the recognition of defects, it was found that the probability of a false positive is in the order of a few percent, the probability of a false negative is in the order of 25%.

The molecular events during nongenotoxic carcinogenesis and their temporal order are poorly understood but thought to include long-lasting perturbations of gene expression. Here, we have investigated the temporal sequence of molecular and pathological perturbations at early stages of phenobarbital (PB) mediated liver tumor promotion in vivo. Molecular profiling (mRNA, microRNA [miRNA], DNA methylation, and proteins) of mouse liver during 13 weeks of PB treatment revealed progressive increases in hepatic expression of long noncoding RNAs and miRNAs originating from the Dlk1-Dio3 imprinted gene cluster, a locus that has recently been associated with stem cell pluripotency in mice and various neoplasms in humans. PB induction of the Dlk1-Dio3 cluster noncoding RNA (ncRNA) Meg3 was localized to glutamine synthetase-positive hypertrophic perivenous hepatocytes, sug- gesting a role for β-catenin signaling in the dysregulation of Dlk1-Dio3 ncRNAs. The carcinogenic relevance of Dlk1-Dio3 locus ncRNA induction was further supported by in vivo genetic dependence on constitutive androstane receptor and β-catenin pathways. Our data identify Dlk1-Dio3 ncRNAs as novel candidate early biomarkers for mouse liver tumor promotion and provide new opportunities for assessing the carcinogenic potential of novel compounds.