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Keywords
Penicillin detection by means of field-effect based sensors: EnFET, capacitive EIS sensor or LAPS?
(2000)
Honarvarfard, Elham ; Gamella, Maria ; Channaveerappa, Devika ; Darie, Costel C. ; Poghossian, Arshak ; Schöning, Michael Josef ; Katz, Evgeny
A graphene-functionalized carbon fiber electrode was modified with adsorbed polyethylenimine to introduce amino functionalities and then with trigonelline and 4-carboxyphenylboronic acid covalently bound to the amino groups. The trigonelline species containing quarterized pyridine groups produced positive charge on the electrode surface regardless of the pH value, while the phenylboronic acid species were neutral below pH 8 and negatively charged above pH 9 (note that their pKa=8.4). The total charge on the monolayer-modified electrode was positive at the neutral pH and negative at pH > 9. Note that 4-carboxyphenylboronic acid was attached to the electrode surface in molar excess to trigonelline, thus allowing the negative charge to dominate on the electrode surface at basic pH. Negatively charged fluorescent dye-labeled insulin (insulin-FITC) was loaded on the modified electrode surface at pH 7.0 due to its electrostatic attraction to the positively charged interface. The local pH in close vicinity to the electrode surface was increased to ca. 9–10 due to consumption of H+ ions upon electrochemical reduction of oxygen proceeding at the potential of −1.0 V (vs. Ag/AgCl) applied on the modified electrode. The process resulted in recharging of the electrode surface to the negative value due to the formation of the negative charge on the phenylboronic acid groups, thus resulting in the electrostatic repulsion of insulin-FITC and stimulating its release from the electrode surface. The insulin release was characterized by fluorescence spectroscopy (using the FITC-labeled insulin), by electrochemical measurements on an iridium oxide, IrOx, electrode and by mass spectrometry. The graphene-functionalized carbon fiber electrode demonstrated significant advantages in the signal-stimulated insulin release comparing with the carbon fiber electrode without the graphene species.
Application of a (bio-)chemical sensor (ISFET) for the detection of physical parameters in liquids
(2003)
Bäcker, Matthias ; Delle, L. ; Poghossian, Arshak ; Biselli, Manfred ; Zang, Werner ; Wagner, P. ; Schöning, Michael Josef
Beging, Stefan ; Poghossian, Arshak ; Schöning, Michael Josef ; Hataihimakul, Sudkanung ; Busch, H. ; Baldsiefen, G. ; Laube, N. ; Kleinen, L. ; Hosseiny, R.
Mourzina, Y. ; Mai, T. ; Poghossian, Arshak ; Ermolenko, Y. ; Yoshinobu, T. ; Vlasov, Y. ; Iwasaki, H. ; Schöning, Michael Josef
Bäcker, Matthias ; Raue, Markus ; Schusser, Sebastian ; Jeitner, C. ; Breuer, L. ; Wagner, P. ; Poghossian, Arshak ; Förster, Arnold ; Mang, Thomas ; Schöning, Michael Josef
Two types of microvalves based on temperature-responsive poly(N-isopropylacrylamide) (PNIPAAm) and pH-responsive poly(sodium acrylate) (PSA) hydrogel films have been developed and tested. The PNIPAAm and PSA hydrogel films were prepared by means of in situ photopolymerization directly inside the fluidic channel of a microfluidic chip fabricated by combining Si and SU-8 technologies. The swelling/shrinking properties and height changes of the PNIPAAm and PSA films inside the fluidic channel were studied at temperatures of deionized water from 14 to 36 °C and different pH values (pH 3–12) of Titrisol buffer, respectively. Additionally, in separate experiments, the lower critical solution temperature (LCST) of the PNIPAAm hydrogel was investigated by means of a differential scanning calorimetry (DSC) and a surface plasmon resonance (SPR) method. Mass-flow measurements have shown the feasibility of the prepared hydrogel films to work as an on-chip integrated temperature- or pH-responsive microvalve capable to switch the flow channel on/off.