Refine
Year of publication
Institute
- INB - Institut für Nano- und Biotechnologien (71) (remove)
Document Type
- Article (65)
- Conference Proceeding (6)
Keywords
- biosensors (3)
- acetoin (2)
- capacitive field-effect sensor (2)
- tobacco mosaic virus (TMV) (2)
- Bacillus atrophaeus (1)
- Bioabsorbable (1)
- Calorimetric gas sensor (1)
- Capacitive field-effect sensor (1)
- Dehydrogenase (1)
- Diaphorase (1)
- Enzymatic biosensor (1)
- Hydrogen peroxide (1)
- Label-free detection (1)
- O2 plasma (1)
- Plant virus (1)
- Polyimide (1)
- Polylactide acid (1)
- Resistive temperature detector (1)
- Silk fibroin (1)
- Simultaneous determination (1)
- Sterilisation process (1)
- TMV adsorption (1)
- Ta₂O₅ gate (1)
- Tobacco mosaic virus (1)
- Tobacco mosaic virus (TMV) (1)
- Zeta potential (1)
- acetoin reductase (1)
- actuator-sensor system (1)
- alcoholic beverages (1)
- amperometric biosensors (1)
- annealing (1)
- bi-enzyme biosensor (1)
- biocompatible (1)
- biocompatible materials (1)
- biodegradabl (1)
- biodegradable electronic devices (1)
- calorimetric gas sensor;hydrogen peroxide;wireless sensor system (1)
- capacitive field-effect biosensor (1)
- capacitive field-effect sensors (1)
- carbon electrodes (1)
- electrolyte-insulator semiconductor sensor (EIS) (1)
- encapsulation materials (1)
- endospores (1)
- enzyme immobilization (1)
- enzyme kinetics (1)
- enzyme-logic gate (1)
- fibroin (1)
- glucose (1)
- hydroxylation (1)
- immobilization (1)
- light-addressable electrode (1)
- light-addressable potentiometric sensor (1)
- microfluidics (1)
- organosilanes (1)
- penicillinase (1)
- plant virus detection (1)
- silanization (1)
- surface functionalization (1)
- urease (1)
The feasibility of light-addressed detection and manipulation of pH gradients inside an electrochemical microfluidic cell was studied. Local pH changes, induced by a light-addressable electrode (LAE), were detected using a light-addressable potentiometric sensor (LAPS) with different measurement modes representing an actuator-sensor system. Biosensor functionality was examined depending on locally induced pH gradients with the help of the model enzyme penicillinase, which had been immobilized in the microfluidic channel. The surface morphology of the LAE and enzyme-functionalized LAPS was studied by scanning electron microscopy. Furthermore, the penicillin sensitivity of the LAPS inside the microfluidic channel was determined with regard to the analyte’s pH influence on the enzymatic reaction rate. In a final experiment, the LAE-controlled pH inhibition of the enzyme activity was monitored by the LAPS.