Refine
Year of publication
Document Type
- Article (30)
- Part of a Book (2)
- Conference Proceeding (2)
- Patent (2)
Language
- English (36) (remove)
Keywords
- Acyl-amino acids (2)
- Aminoacylase (2)
- Bacillaceae (2)
- Biotechnological application (2)
- Subtilases (2)
- Subtilisin (2)
- acetoin (2)
- Acylation (1)
- Alginate beads (1)
- Alkalihalobacillus okhensis (1)
- Biocatalysis (1)
- Biosurfactants (1)
- Broad pH spectrum (1)
- Chaperone (1)
- Chaperone co-expression (1)
- Detergent protease (1)
- Halotolerant protease (1)
- Inclusion bodies (1)
- Stenotrophomonas maltophilia (1)
- Streptomyces griseus (1)
An amperometric enzyme biosensor has been applied for the detection of adrenaline. The adrenaline biosensor has been prepared by modification of an oxygen electrode with the enzyme laccase that operates at a broad pH range between pH 3.5 to pH 8. The enzyme molecules were immobilized via cross-linking with glutaraldehyde. The sensitivity of the developed adrenaline biosensor in different pH buffer solutions has been studied.
An amperometric biosensor using a substrate recycling principle was realized for the detection of low adrenaline concentrations (1 nM) by measurements in phosphate buffer and Ringer’s solution at pH 6.5 and pH 7.4, respectively. In proof-of-concept experiments, a Boolean logic-gate principle has been applied to develop a digital adrenaline biosensor based on an enzyme AND logic gate. The obtained results demonstrate that the developed digital biosensor is capable for a rapid qualitative determination of the presence/absence of adrenaline in a YES/NO statement. Such digital biosensor could be used in clinical diagnostics for the control of a correct insertion of a catheter in the adrenal veins during adrenal venous-sampling procedure.