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Keywords
Molinnus, Denise ; Bäcker, Matthias ; Siegert, Petra ; Willenberg, H. ; Poghossian, Arshak ; Keusgen, M. ; Schöning, Michael Josef
An amperometric enzyme biosensor has been applied for the detection of adrenaline. The adrenaline biosensor has been prepared by modification of an oxygen electrode with the enzyme laccase that operates at a broad pH range between pH 3.5 to pH 8. The enzyme molecules were immobilized via cross-linking with glutaraldehyde. The sensitivity of the developed adrenaline biosensor in different pH buffer solutions has been studied.
Wiegand, Sandra ; Voigt, Birgit ; Albrecht, Dirk ; Bongaerts, Johannes ; Evers, Stefan ; Hecker, Michael ; Daniel, Rolf ; Liesegang, Heiko
Raab, Monika ; Kappel, Sven ; Krämer, Andrea ; Sanhaji, Mourad ; Matthess, Yves ; Kurunci-Csacsko, Elisabeth ; Calzada-Wack, Julia ; Rathkolb, Birgit ; Rosman, Jan ; Adler, Thure ; Busch, Dirk H. ; Esposito, Irene ; Fuchs, Helmut ; Gailus-Durner, Valérie ; Klingenspor, Martin ; Wolf, Eckhard ; Sänger, Nicole ; Prinz, Florian ; Hrabe de Angelis, Martin ; Seibler, Jost ; Yuan, Juping ; Bergmann, Martin ; Knecht, Rainald ; Kreft, Bertolt ; Strebhardt, Klaus
Guo, Yuanyuan ; Seki, Kosuke ; Miyamoto, Ko-ichiro ; Wagner, Torsten ; Schöning, Michael Josef ; Yoshinobu, Tatsuo
A novel photoexcitation method for the light-addressable potentiometric sensor (LAPS) realized a higher spatial resolution of chemical imaging. In this method, a modulated light probe, which generates the alternating photocurrent signal, is surrounded by a ring of constant light, which suppresses the lateral diffusion of photocarriers by enhancing recombination. A device simulation verified that a higher spatial resolution could be obtained by adjusting the gap between the modulated and constant light. It was also found that a higher intensity and a longer wavelength of constant light was more effective. However, there exists a tradeoff between the spatial resolution and the amplitude of the photocurrent, and thus, the signal-to-noise ratio. A tilted incidence of constant light was applied, which could achieve even higher resolution with a smaller loss of photocurrent.
Schusser, Sebastian ; Bäcker, Matthias ; Krischer, M. ; Wenzel, L. ; Leinhos, Marcel ; Poghossian, Arshak ; Biselli, Manfred ; Wagner, P. ; Schöning, Michael Josef
A semiconductor field-effect device has been used for an enzymatically catalyzed degradation of biopolymers for the first time. This novel technique is capable to monitor the degradation process of multiple samples in situ and in real-time. As model system, the degradation of the biopolymer poly(D, L-lactic acid) has been monitored in the degradation medium containing the enzyme lipase from Rhizomucor miehei. The obtained results demonstrate the potential of capacitive field-effect sensors for degradation studies of biodegradable polymers.
Huck, Christina ; Poghossian, Arshak ; Bäcker, Matthias ; Reisert, Steffen ; Schubert, J. ; Zander, W. ; Begoyan, V. K. ; Buniatyan, V. V. ; Schöning, Michael Josef
High-k perovskite oxide of barium strontium titanate (BST) represents a very attractive multi-functional transducer material for the development of (bio-)chemical sensors for liquids. In this work, BST films have been applied as a sensitive transducer material for a label-free detection of adsorbed charged macromolecules (positively charged polyelectrolytes) and concentration of hydrogen peroxide vapor as well as protection insulator layer for a contactless electrolyte-conductivity sensor. The experimental results of characterization of individual sensors are presented. Special emphasis is devoted towards the development of a capacitively-coupled contactless electrolyte-conductivity sensor.
Bronder, Thomas ; Wu, Chunsheng ; Poghossian, Arshak ; Werner, Frederik ; Keusgen, M. ; Schöning, Michael Josef
Light-addressable potentiometric sensors (LAPS) consisting of a p-Si-SiO2 and p-Si-SiO2-Au structure, respectively, have been tested for a label-free electrical detection of DNA (deoxyribonucleic acid) hybridization. Three different strategies for immobilizing single-stranded probe DNA (ssDNA) molecules on a LAPS surface have been studied and compared: (a) immobilization of thiol-modified ssDNA on the patterned Au surface via gold-thiol bond, (b) covalent immobilization of amino-modified ssDNA onto the SiO2 surface functionalized with 3-aminopropyltriethoxysilane and (c) layer-by-layer adsorption of negatively charged ssDNA on a positively charged weak polyelectrolyte layer of poly(allylamine hydrochloride).