610 Medizin und Gesundheit
Refine
Has Fulltext
- yes (8)
Language
- English (8)
Document Type
- Conference Proceeding (8) (remove)
Keywords
- Hämoglobin (3)
- Erythrozyt (2)
- Kohlenstofffaser (2)
- Lipopolysaccharide (2)
- celldrum technology (2)
- lipopolysaccharides (2)
- Adsorption (1)
- Bakterien (1)
- Clusterion (1)
- Endothelzelle (1)
Is part of the Bibliography
- no (8)
Summary and Conclusions PCIs were clearly effective in terms of their antibacterial effects with the strains tested. This efficacy increased with the time the bacteries were exposed to PCIs. The bactericidal action has proved to be irreversible. PCIs were significantly less effective in shadowed areas. PCI exposure caused multiple protein damages as observed in SDS PAGE studies. There was no single but multiple molecular mechanism causing the bacterial death.
Mechanical stimulation of the cells resulted in evident changes in the cell morphology, protein composition and gene expression. Microscopically, additional formation of stress fibers accompanied by cell re-arrangements in a monolayer was observed. Also, significant activation of p53 gene was revealed as compared to control. Interestingly, the use of CellTech membrane coating induced cell death after mechanical stress had been applied. Such an effect was not detected when fibronectin had been used as an adhesion substrate.
As a deduction from these results, we can conclude that proteins mainly in vitro, denaturate totally at a temperature between 57°C -62°C, and they also affected by NO and different ions types. In which mainly, NO cause earlier protein denaturation, which means that, NO has a destabilizing effect on proteins, and also different ions will alter the protein denaturation in which, some ions will cause earlier protein denaturation while others not.