Conference Proceeding
Refine
Year of publication
- 2006 (94) (remove)
Document Type
- Conference Proceeding (94) (remove)
Keywords
- Biosensor (23)
- Bauingenieurwesen (2)
- Bauplanung (2)
- CAD (2)
- biosensor (2)
- civil engineering (2)
- 3-nitrofluoranthene (1)
- Aachen / Fachhochschule Aachen / Fachbereich Bauingenieurwesen (1)
- Absolvententreffen (1)
- Abwasser (1)
- Alfha.net (1)
- Alumni (1)
- Anlagenwirtschaft (1)
- Asteroid Deflection (1)
- BTEX compounds (1)
- Bauführung (1)
- Bauherrenvertretung (1)
- Bauleitung (1)
- Baurevision (1)
- Berufsperspektiven (1)
Institute
- Fachbereich Medizintechnik und Technomathematik (34)
- Fachbereich Bauingenieurwesen (15)
- Fachbereich Maschinenbau und Mechatronik (14)
- INB - Institut für Nano- und Biotechnologien (13)
- Fachbereich Energietechnik (10)
- Fachbereich Luft- und Raumfahrttechnik (9)
- Fachbereich Wirtschaftswissenschaften (5)
- Solar-Institut Jülich (4)
- IfB - Institut für Bioengineering (3)
- Nowum-Energy (2)
- ECSM European Center for Sustainable Mobility (1)
- Fachbereich Chemie und Biotechnologie (1)
- Fachbereich Elektrotechnik und Informationstechnik (1)
- Fachbereich Gestaltung (1)
In this paper, methods of sample preparation for potentiometric measurement of phenylalanine are presented. Basing on the spectrophotometric measurements of phenylalanine, the concentrations of reagents of the enzymatic reaction (10 mM L-Phe, 0,4 mM NAD+, 2U L-PheDH) were determined. Then, the absorption spectrum of the reaction product, NADH, was monitored (maximum peak at 340 nm). The results obtained by the spectrophotometric method were compared with the results obtained by the colourimetry, using pH indicators. The above-mentioned two methods will be used as references for potentiometric measurements of phenylalanine concentration.