Refine
Year of publication
- 2024 (93)
- 2023 (186)
- 2022 (234)
- 2021 (229)
- 2020 (232)
- 2019 (301)
- 2018 (252)
- 2017 (255)
- 2016 (260)
- 2015 (284)
- 2014 (280)
- 2013 (285)
- 2012 (313)
- 2011 (310)
- 2010 (331)
- 2009 (341)
- 2008 (290)
- 2007 (271)
- 2006 (276)
- 2005 (263)
- 2004 (286)
- 2003 (218)
- 2002 (232)
- 2001 (210)
- 2000 (234)
- 1999 (232)
- 1998 (236)
- 1997 (214)
- 1996 (200)
- 1995 (192)
- 1994 (174)
- 1993 (154)
- 1992 (144)
- 1991 (100)
- 1990 (108)
- 1989 (110)
- 1988 (103)
- 1987 (105)
- 1986 (81)
- 1985 (83)
- 1984 (75)
- 1983 (70)
- 1982 (57)
- 1981 (54)
- 1980 (61)
- 1979 (58)
- 1978 (52)
- 1977 (32)
- 1976 (30)
- 1975 (28)
- 1974 (17)
- 1973 (12)
- 1972 (17)
- 1971 (11)
- 1970 (2)
- 1969 (2)
- 1968 (2)
- 1967 (1)
- 1963 (1)
Institute
- Fachbereich Medizintechnik und Technomathematik (1928)
- Fachbereich Elektrotechnik und Informationstechnik (1150)
- Fachbereich Wirtschaftswissenschaften (1119)
- Fachbereich Energietechnik (1066)
- Fachbereich Chemie und Biotechnologie (892)
- Fachbereich Maschinenbau und Mechatronik (801)
- Fachbereich Luft- und Raumfahrttechnik (769)
- Fachbereich Bauingenieurwesen (664)
- IfB - Institut für Bioengineering (627)
- INB - Institut für Nano- und Biotechnologien (585)
Has Fulltext
- no (9284) (remove)
Language
Document Type
- Article (5518)
- Conference Proceeding (1413)
- Book (1057)
- Part of a Book (555)
- Patent (174)
- Bachelor Thesis (169)
- Report (82)
- Doctoral Thesis (79)
- Conference: Meeting Abstract (75)
- Other (67)
Keywords
- Illustration (10)
- Nachhaltigkeit (10)
- Corporate Design (9)
- Erscheinungsbild (8)
- Gamification (8)
- Redesign (7)
- Animation (6)
- Datenschutz (6)
- Deutschland (6)
- Digitalisierung (6)
In the present work, surface functionalization of different sensor materials was studied. Organosilanes are well known to serve as coupling agent for biomolecules or cells on inorganic materials. 3-aminopropyltriethoxysilane (APTES) was used to attach microbiological spores time to an interdigitated sensor surface. The functionality and physical properties of APTES were studied on isolated sensor materials, namely silicon dioxide (SiO2) and platinum (Pt) as well as the combined material on sensor level. A predominant immobilization of spores could be demonstrated on SiO2 surfaces. Additionally, the impedance signal of APTES-functionalized biosensor chips has been investigated.
Optimization of the immobilization of bacterial spores on glass substrates with organosilanes
(2016)
Spores can be immobilized on biosensors to function as sensitive recognition elements. However, the immobilization can affect the sensitivity and reproducibility of the sensor signal. In this work, three different immobilization strategies with organosilanes were optimized and characterized to immobilize Bacillus atrophaeus spores on glass substrates. Five different silanization parameters were investigated: nature of the solvent, concentration of the silane, silanization time, curing process, and silanization temperature. The resulting silane layers were resistant to a buffer solution (e.g., Ringer solution) with a polysorbate (e.g., Tween®80) and sonication.
Prior to immobilization of biomolecules or cells onto biosensor surfaces, the surface must be physically or chemically activated for further functionalization. Organosilanes are a versatile option as they facilitate the immobilization through their terminal groups and also display self-assembly. Incorporating hydroxyl groups is one of the important methods for primary immobilization. This can be done, for example, with oxygen plasma treatment. However, this treatment can affect the performance of the biosensors and this effect is not quite well understood for surface functionalization. In this work, the effect of O2 plasma treatment on EIS sensors was investigated by means of electrochemical characterizations: capacitance–voltage (C–V) and constant capacitance (ConCap) measurements. After O2 plasma treatment, the potential of the EIS sensor dramatically shifts to a more negative value. This was successfully reset by using an annealing process.