Article
Refine
Year of publication
- 2024 (5)
- 2023 (15)
- 2022 (14)
- 2021 (11)
- 2020 (10)
- 2019 (7)
- 2018 (12)
- 2017 (11)
- 2016 (19)
- 2015 (20)
- 2014 (25)
- 2013 (24)
- 2012 (15)
- 2011 (22)
- 2010 (15)
- 2009 (18)
- 2008 (10)
- 2007 (14)
- 2006 (13)
- 2005 (12)
- 2004 (8)
- 2003 (8)
- 2002 (15)
- 2001 (16)
- 2000 (9)
- 1999 (10)
- 1998 (23)
- 1997 (15)
- 1996 (8)
- 1995 (28)
- 1994 (22)
- 1993 (9)
- 1992 (20)
- 1991 (8)
- 1990 (14)
- 1989 (15)
- 1988 (13)
- 1987 (12)
- 1986 (10)
- 1985 (7)
- 1984 (7)
- 1983 (9)
- 1982 (3)
- 1981 (2)
- 1980 (3)
- 1979 (8)
- 1978 (2)
- 1976 (1)
- 1975 (3)
- 1972 (1)
- 1971 (2)
Institute
- Fachbereich Chemie und Biotechnologie (603) (remove)
Document Type
- Article (603) (remove)
Keywords
- Heparin (3)
- Bacillaceae (2)
- Biotechnological application (2)
- Butanol (2)
- Chemometrics (2)
- IR spectroscopy (2)
- NMR spectroscopy (2)
- Principal component analysis (2)
- Standardization (2)
- Subtilases (2)
Poly(N-isopropylacrylamide) (PNIPAAm) hydrogel films with incorporated graphene oxide (GO) were developed and tested as light-stimulated actuators. GO dispersions were synthesized via Hummers method and characterized toward their optical properties and photothermal energy conversion. The hydrogels were prepared by means of photopolymerization. In addition, the influence of GO within the hydrogel network on the lower critical solution temperature (LCST) was investigated by differential scanning calorimetry (DSC). The optical absorbance and the response to illumination were determined as a function of GO concentration for thin hydrogel films. A proof of principle for the stimulation with light was performed.
A new microfluidic assembly method for semiconductor-based biosensors using 3D-printing technologies was proposed for a rapid and cost-efficient design of new sensor systems. The microfluidic unit is designed and printed by a 3D-printer in just a few hours and assembled on a light-addressable potentiometric sensor (LAPS) chip using a photo resin. The cell growth curves obtained from culturing cells within microfluidics-based LAPS systems were compared with cell growth curves in cell culture flasks to examine biocompatibility of the 3D-printed chips. Furthermore, an optimal cell culturing within microfluidics-based LAPS chips was achieved by adjusting the fetal calf serum concentrations of the cell culture medium, an important factor for the cell proliferation.
BACKGROUND
Currently, several techniques exist for the downstream processing of protein, phytic acid and sinapic acid from rapeseed and rapeseed meal, but no technique has been developed to separate all of the components in one process. In this work, two new downstream processing strategies focusing on recovering sinapic acid, phytic acid and protein from rapeseed meal were established.
RESULTS
The sinapic acid content was enhanced by a factor of 4.5 with one method and 5.1 with the other. The isolation of sinapic acid was accomplished using a zeolite-based adsorbent with high adsorptive and optimal desorption characteristics. Phytic acid was isolated using the anion-exchange resin Purolite A200®. In addition, the processes resulted in two separated protein fractions. The ratios of globulin and albumin ratio to the total protein were 59.2% and 40.1%, respectively. The steps were then combined in two different ways: (a) a ‘sequential process’ using the zeolite and A200 in batch processes; and (b) a ‘parallel process’ using only A200 in a chromatographic system to separate all of the compounds.
CONCLUSIONS
It can be concluded that isolation of all three components was possible in both processes. These could enhance the added value of current processes using rapeseed meal as a protein source. © 2015 Society of Chemical Industry