Refine
Year of publication
Document Type
- Patent (36)
- Article (26)
- Part of a Book (2)
- Report (2)
- Conference Proceeding (1)
Keywords
- Bacillaceae (2)
- Biotechnological application (2)
- Subtilases (2)
- Subtilisin (2)
- Alginate beads (1)
- Alkalihalobacillus okhensis (1)
- Broad pH spectrum (1)
- Detergent protease (1)
- Halotolerant protease (1)
- Stenotrophomonas maltophilia (1)
- Streptomyces griseus (1)
- Streptomyces lividans (1)
- acetoin (1)
- acetoin reductase (1)
- alcoholic beverages (1)
- biosensors (1)
- capacitive field-effect sensors (1)
- detergent protease (1)
- halotolerant protease (1)
- high-alkaline subtilisin (1)
Institute
- Fachbereich Chemie und Biotechnologie (67) (remove)
The enantioselective synthesis of α-hydroxy ketones and vicinal diols is an intriguing field because of the broad applicability of these molecules. Although, butandiol dehydrogenases are known to play a key role in the production of 2,3-butandiol, their potential as biocatalysts is still not well studied. Here, we investigate the biocatalytic properties of the meso-butanediol dehydrogenase from Bacillus licheniformis DSM 13T (BlBDH). The encoding gene was cloned with an N-terminal StrepII-tag and recombinantly overexpressed in E. coli. BlBDH is highly active towards several non-physiological diketones and α-hydroxyketones with varying aliphatic chain lengths or even containing phenyl moieties. By adjusting the reaction parameters in biotransformations the formation of either the α-hydroxyketone intermediate or the diol can be controlled.
α-hydroxy ketones (HK) and 1,2-diols are important building blocks for fine chemical synthesis. Here, we describe the R-selective 2,3-butanediol dehydrogenase from B. clausii DSM 8716ᵀ (BcBDH) that belongs to the metal-dependent medium chain dehydrogenases/reductases family (MDR) and catalyzes the selective asymmetric reduction of prochiral 1,2-diketones to the corresponding HK and, in some cases, the reduction of the same to the corresponding 1,2-diols. Aliphatic diketones, like 2,3-pentanedione, 2,3-hexanedione, 5-methyl-2,3-hexanedione, 3,4-hexanedione and 2,3-heptanedione are well transformed. In addition, surprisingly alkyl phenyl dicarbonyls, like 2-hydroxy-1-phenylpropan-1-one and phenylglyoxal are accepted, whereas their derivatives with two phenyl groups are not substrates. Supplementation of Mn²⁺ (1 mM) increases BcBDH's activity in biotransformations. Furthermore, the biocatalytic reduction of 5-methyl-2,3-hexanedione to mainly 5-methyl-3-hydroxy-2-hexanone with only small amounts of 5-methyl-2-hydroxy-3-hexanone within an enzyme membrane reactor is demonstrated.