Refine
Year of publication
Document Type
- Article (3149)
- Conference Proceeding (1016)
- Part of a Book (184)
- Book (144)
- Doctoral Thesis (30)
- Patent (25)
- Other (9)
- Report (9)
- Preprint (4)
- Poster (3)
- Talk (3)
- Master's Thesis (2)
- Working Paper (2)
- Bachelor Thesis (1)
- Contribution to a Periodical (1)
- Habilitation (1)
Language
- English (4583) (remove)
Has Fulltext
- no (4583) (remove)
Keywords
- Gamification (6)
- avalanche (6)
- Earthquake (5)
- Enterprise Architecture (5)
- MINLP (5)
- solar sail (5)
- Diversity Management (4)
- Energy storage (4)
- Engineering optimization (4)
- LAPS (4)
- Natural language processing (4)
- Papierkunst (4)
- Power plants (4)
- Seismic design (4)
- field-effect sensor (4)
- frequency mixing magnetic detection (4)
- hydrogen (4)
- metal structure (4)
- snow (4)
- steel (4)
Institute
- Fachbereich Medizintechnik und Technomathematik (1545)
- Fachbereich Elektrotechnik und Informationstechnik (686)
- IfB - Institut für Bioengineering (560)
- Fachbereich Energietechnik (552)
- INB - Institut für Nano- und Biotechnologien (532)
- Fachbereich Chemie und Biotechnologie (522)
- Fachbereich Luft- und Raumfahrttechnik (463)
- Fachbereich Maschinenbau und Mechatronik (261)
- Fachbereich Wirtschaftswissenschaften (196)
- Solar-Institut Jülich (160)
- Fachbereich Bauingenieurwesen (146)
- ECSM European Center for Sustainable Mobility (75)
- MASKOR Institut für Mobile Autonome Systeme und Kognitive Robotik (62)
- Fachbereich Gestaltung (24)
- Nowum-Energy (24)
- Institut fuer Angewandte Polymerchemie (23)
- Sonstiges (21)
- Fachbereich Architektur (20)
- Freshman Institute (18)
- Kommission für Forschung und Entwicklung (18)
- ZHQ - Bereich Hochschuldidaktik und Evaluation (8)
- IMP - Institut für Mikrowellen- und Plasmatechnik (3)
- IaAM - Institut für angewandte Automation und Mechatronik (2)
- Arbeitsstelle fuer Hochschuldidaktik und Studienberatung (1)
- Digitalisierung in Studium & Lehre (1)
- FH Aachen (1)
Investigation of TRPV1 loss-of-function phenotypes in transgenic shRNA expressing and knockout mice
(2008)
The contribution of altered post-transcriptional gene silencing to the development of insulin resistance and type 2 diabetes mellitus so far remains elusive. Here, we demonstrate that expression of microRNA (miR)-143 and 145 is upregulated in the liver of genetic and dietary mouse models of obesity. Induced transgenic overexpression of miR-143, but not miR-145, impairs insulin-stimulated AKT activation and glucose homeostasis. Conversely, mice deficient for the miR-143–145 cluster are protected from the development of obesity-associated insulin resistance. Quantitative-mass-spectrometry-based analysis of hepatic protein expression in miR-143-overexpressing mice revealed miR-143-dependent downregulation of oxysterol-binding-protein-related protein (ORP) 8. Reduced ORP8 expression in cultured liver cells impairs the ability of insulin to induce AKT activation, revealing an ORP8-dependent mechanism of AKT regulation. Our experiments provide direct evidence that dysregulated post-transcriptional gene silencing contributes to the development of obesity-induced insulin resistance, and characterize the miR-143–ORP8 pathway as a potential target for the treatment of obesity-associated diabetes.