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Keywords
Jablonski, Melanie ; Koch, Claudia ; Bronder, Thomas ; Poghossian, Arshak ; Wege, Christina ; Schöning, Michael Josef
The chemical imaging sensor is a semiconductor-based chemical sensor that can visualize the spatial distribution of specific ions on the sensing surface. The conventional chemical imaging system based on the light-addressable potentiometric sensor (LAPS), however, required a long time to obtain a chemical image, due to the slow mechanical scan of a single light beam. For high-speed imaging, a plurality of light beams modulated at different frequencies can be employed to measure the ion concentrations simultaneously at different locations on the sensor plate by frequency division multiplex (FDM). However, the conventional measurement geometry of back-side illumination limited the bandwidth of the modulation frequency required for FDM measurement, because of the low-pass filtering characteristics of carrier diffusion in the Si substrate. In this study, a high-speed chemical imaging system based on front-side-illuminated LAPS was developed, which achieved high-speed spatiotemporal recording of pH change at a rate of 70 frames per second.
Ingebrandt, S. ; Han, Y. ; Nakamura, F. ; Poghossian, Arshak ; Schöning, Michael Josef ; Offenhäusser, A.
Immel, Timo ; Grützke, Martin ; Späte, Anne-Katrin ; Groth, Ulrich ; Öhlschläger, Peter ; Huhn, Thomas
Chelate stabilization of a titanium(IV)–salan alkoxide by ligand exchange with 2,6-pyridinedicarboxylic acid (dipic) resulted in heptacoordinate complex 3 which is not redox-active, stable on silica gel and has increased aqueous stability. 3 is highly toxic in HeLa S3 and Hep G2 and has enhanced antitumor efficacy in a mouse cervical-cancer model.
A variety of transition metals, e.g., copper, zinc, cadmium, lead, etc. are widely used in industry as components for wires, coatings, alloys, batteries, paints and so on. The inevitable presence of transition metals in industrial processes implies the ambition of developing a proper analytical technique for their adequate monitoring. Most of these elements, especially lead and cadmium, are acutely toxic for biological organisms. Quantitative determination of these metals at low activity levels in different environmental and industrial samples is therefore a vital task. A promising approach to achieve an at-side or on-line monitoring on a miniaturized and cost efficient way is the combination of a common potentiometric sensor array with heavy metal-sensitive thin-film materials, like chalcogenide glasses and polymeric materials, respectively.
Iken, Heiko ; Bronder, Thomas ; Goretzki, Alexander ; Kriesel, Jana ; Ahlborn, Kristina ; Gerlach, Frank ; Vonau, Winfried ; Zander, Willi ; Schubert, Jürgen ; Schöning, Michael Josef
Iken, Heiko ; Ahlborn, Kristina ; Gerlach, Frank ; Vonau, Winfried ; Zander, Wilhelm ; Schubert, Jürgen P. ; Schöning, Michael Josef
Huck, Christina ; Schiffels, Johannes ; Herrera, Cony N. ; Schelden, Maximilian ; Selmer, Thorsten ; Poghossian, Arshak ; Baumann, Marcus ; Wagner, Patrick ; Schöning, Michael Josef
Living cells are complex biological systems transforming metabolites taken up from the surrounding medium. Monitoring the responses of such cells to certain substrate concentrations is a challenging task and offers possibilities to gain insight into the vitality of a community influenced by the growth environment. Cell-based sensors represent a promising platform for monitoring the metabolic activity and thus, the “welfare” of relevant organisms. In the present study, metabolic responses of the model bacterium Escherichia coli in suspension, layered onto a capacitive field-effect structure, were examined to pulses of glucose in the concentration range between 0.05 and 2 mM. It was found that acidification of the surrounding medium takes place immediately after glucose addition and follows Michaelis–Menten kinetic behavior as a function of the glucose concentration. In future, the presented setup can, therefore, be used to study substrate specificities on the enzymatic level and may as well be used to perform investigations of more complex metabolic responses. Conclusions and perspectives highlighting this system are discussed.