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In this study, polyelectrolyte-modified field-effect-based electrolyte-insulator-semiconductor (EIS) devices have been used for the label-free electrical detection of double-stranded deoxyribonucleic acid (dsDNA)molecules. The sensor-chip functionalization with a positively charged polyelectrolyte layer provides the possibility of direct adsorptive binding of negatively charged target DNA oligonucleotides onto theSiO2-chip surface.EIS sensors can be utilized as a tool to detect surface-charge changes; the electrostatic adsorption of oligonucleotides onto the polyelectrolyte layer leads to a measureable surface-potential change. Signals of 39mV have been recorded after the incubation with the oligonucleotide solution. Besides the electrochemical experiments, the successful adsorption of dsDNA onto the polyelectrolyte layer has been verified via fluorescence microscopy. The presented results demonstrate that the signal recording of EISchips, which are modified with a polyelectrolyte layer, canbe used as a favorable approach for a fast, cheap and simple detection method for dsDNA.
In the present work, surface functionalization of different sensor materials was studied. Organosilanes are well known to serve as coupling agent for biomolecules or cells on inorganic materials. 3-aminopropyltriethoxysilane (APTES) was used to attach microbiological spores time to an interdigitated sensor surface. The functionality and physical properties of APTES were studied on isolated sensor materials, namely silicon dioxide (SiO2) and platinum (Pt) as well as the combined material on sensor level. A predominant immobilization of spores could be demonstrated on SiO2 surfaces. Additionally, the impedance signal of APTES-functionalized biosensor chips has been investigated.
Assessment of RF Safety of Transmit Coils at 7 Tesla by Experimental and Numerical Procedures (490.)
(2012)
This work presents a methodology for automated
damage-sensitive feature extraction and anomaly
detection under multivariate operational variability
for in-flight assessment of wings. The
method uses a passive excitation approach, i. e.
without the need for artificial actuation. The
modal system properties (natural frequencies and
damping ratios) are used as damage-sensitive
features. Special emphasis is placed on the use
of Fiber Bragg Grating (FBG) sensing technology
and the consideration of Operational and
Environmental Variability (OEV). Measurements
from a wind tunnel investigation with a composite
cantilever equipped with FBG and piezoelectric
sensors are used to successfully detect an impact
damage. In addition, the feasibility of damage
localisation and severity estimation is evaluated
based on the coupling found between damageand
OEV-induced feature changes.