Refine
Author
- Michael Josef Schöning (551) (remove)
Year of publication
Document Type
- Article (491)
- Conference Proceeding (46)
- Part of a Book (10)
- Book (2)
- Other (2)
Language
- English (551) (remove)
Keywords
- Biosensor (7)
- LAPS (4)
- field-effect sensor (4)
- hydrogen peroxide (4)
- Field-effect sensor (3)
- Label-free detection (3)
- Light-addressable potentiometric sensor (3)
- biosensors (3)
- capacitive field-effect sensor (3)
- tobacco mosaic virus (TMV) (3)
- Bacillus atrophaeus (2)
- Calorimetric gas sensor (2)
- Capacitive field-effect sensor (2)
- Hydrogen peroxide (2)
- Raman spectroscopy (2)
- Tobacco mosaic virus (TMV) (2)
- acetoin (2)
- capacitive field-effect sensors (2)
- gold nanoparticles (2)
- light-addressable potentiometric sensor (2)
Institute
Tobias Karschuck
; Arshak Poghossian
; Joey Ser
; Astghik Tsokolakyan
; Stefan Achtsnicht
; Patrick Wagner
; Michael Josef Schöning
Electrolyte-insulator-semiconductor capacitors (EISCAP) belong to field-effect sensors having an attractive transducer architecture for constructing various biochemical sensors. In this study, a capacitive model of enzyme-modified EISCAPs has been developed and the impact of the surface coverage of immobilized enzymes on its capacitance-voltage and constant-capacitance characteristics was studied theoretically and experimentally. The used multicell arrangement enables a multiplexed electrochemical characterization of up to sixteen EISCAPs. Different enzyme coverages have been achieved by means of parallel electrical connection of bare and enzyme-covered single EISCAPs in diverse combinations. As predicted by the model, with increasing the enzyme coverage, both the shift of capacitance-voltage curves and the amplitude of the constant-capacitance signal increase, resulting in an enhancement of analyte sensitivity of the EISCAP biosensor. In addition, the capability of the multicell arrangement with multi-enzyme covered EISCAPs for sequentially detecting multianalytes (penicillin and urea) utilizing the enzymes penicillinase and urease has been experimentally demonstrated and discussed.