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Year of publication
- 2014 (93) (remove)
Institute
- Fachbereich Medizintechnik und Technomathematik (39)
- INB - Institut für Nano- und Biotechnologien (24)
- Fachbereich Chemie und Biotechnologie (23)
- IfB - Institut für Bioengineering (11)
- Fachbereich Elektrotechnik und Informationstechnik (10)
- Fachbereich Wirtschaftswissenschaften (5)
- Fachbereich Luft- und Raumfahrttechnik (4)
- Fachbereich Energietechnik (3)
- Solar-Institut Jülich (3)
- Sonstiges (3)
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- no (93)
Language
- English (93) (remove)
Document Type
- Article (93) (remove)
Keywords
Akimbekov, Nuraly S. ; Digel, Ilya ; O´Heras, C. ; Tastambek, K.T. ; Savitskaya, I.S. ; Ualyeva, P.S. ; Mansurov, Z.A. ; Zhubanova, A.A.
The scope of this study is the measurement of endotoxin adsorption rate for carbonized rice husk. It showed good adsorption properties for LPS. During the batch experiments, several techniques were used and optimized for improving the material’s adsorption behavior. Also, with the results obtained it was possible to differentiate the materials according to their adsorption capacity and kinetic characteristics.
Winckler, Silvia ; Krueger, Rolf ; Schnitzler, Thomas ; Zang, Werner ; Fischer, Rainer ; Biselli, Manfred
Biopharmaceuticals such as antibodies are produced in cultivated mammalian cells, which must be monitored to comply with good manufacturing practice. We, therefore, developed a fully automated system comprising a specific exhaust gas analyzer, inline analytics and a corresponding algorithm to precisely determine the oxygen uptake rate, carbon dioxide evolution rate, carbon dioxide transfer rate, transfer quotient and respiratory quotient without interrupting the ongoing cultivation, in order to assess its reproducibility. The system was verified using chemical simulation experiments and was able to measure the respiratory activity of hybridoma cells and DG44 cells (derived from Chinese hamster ovary cells) with satisfactory results at a minimum viable cell density of ~2.0 × 10⁵ cells ml⁻¹. The system was suitable for both batch and fed-batch cultivations in bubble-aerated and membrane-aerated reactors, with and without the control of pH and dissolved oxygen.