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Year of publication
Institute
- Fachbereich Chemie und Biotechnologie (472) (remove)
Language
- English (472) (remove)
Document Type
- Article (472) (remove)
Keywords
- Heparin (3)
- Bacillaceae (2)
- Biotechnological application (2)
- Butanol (2)
- Chemometrics (2)
- IR spectroscopy (2)
- NMR spectroscopy (2)
- Principal component analysis (2)
- Standardization (2)
- Subtilases (2)
Whitehead, Mark ; Öhlschläger, Peter ; Almajhdi, Fahad N. ; Alloza, Leonor ; Marzábal, Pablo ; Meyers, Ann E. ; Hitzeroth, Inga I. ; Rybicki, Edward P.
Danho, Waleed ; Naithani, Vinod K. ; Sasaki, André N. ; Föhles, Joseph ; Berndt, Heinz ; Büllesbach, Erika E. ; Zahn, H.
Breuer, Lars ; Raue, Markus ; Strobel, M. ; Mang, Thomas ; Schöning, Michael Josef ; Thoelen, R. ; Wagner, Torsten
Abstractauthoren Graphene oxide (GO) nanoparticles were incorporated in temperature-sensitive Poly(N-isopropylacrylamide) (PNIPAAm) hydrogels. The nanoparticles increase the light absorption and convert light energy into heat efficiently. Thus, the hydrogels with GO can be stimulated spatially resolved by illumination as it was demonstrated by IR thermography. The temporal progression of the temperature maximum was detected for different concentrations of GO within the polymer network. Furthermore, the compatibility of PNIPAAm hydrogels with GO and cell cultures was investigated. For this purpose, culture medium was incubated with hydrogels containing GO and the viability and morphology of chinese hamster ovary (CHO) cells was examined after several days of culturing in presence of this medium.
Biomass from various types of organic waste was tested for possible use in hydrogen production. The composition consisted of lignified samples, green waste, and kitchen scraps such as fruit and vegetable peels and leftover food. For this purpose, the enzymatic pretreatment of organic waste with a combination of five different hydrolytic enzymes (cellulase, amylase, glucoamylase, pectinase and xylase) was investigated to determine its ability to produce hydrogen (H2) with the hydrolyzate produced here. In course, the anaerobic rod-shaped bacterium T. neapolitana was used for H2 production. First, the enzymes were investigated using different substrates in preliminary experiments. Subsequently, hydrolyses were carried out using different types of organic waste. In the hydrolysis carried out here for 48 h, an increase in glucose concentration of 481% was measured for waste loads containing starch, corresponding to a glucose concentration at the end of hydrolysis of 7.5 g·L−1. In the subsequent set fermentation in serum bottles, a H2 yield of 1.26 mmol H2 was obtained in the overhead space when Terrific Broth Medium with glucose and yeast extract (TBGY medium) was used. When hydrolyzed organic waste was used, even a H2 yield of 1.37 mmol could be achieved in the overhead space. In addition, a dedicated reactor system for the anaerobic fermentation of T. neapolitana to produce H2 was developed. The bioreactor developed here can ferment anaerobically with a very low loss of produced gas. Here, after 24 h, a hydrogen concentration of 83% could be measured in the overhead space.
Lempiäinen, Harri ; Couttet, Philippe ; Bolognani, Federico ; Müller, Arne ; Dubost, Valérie ; Luisier, Raphaëlle ; Rio-Espinola, Alberto del ; Vitry, Veronique ; Unterberger, Elif B. ; Thomson, John P. ; Treindl, Fridolin ; Metzger, Ute ; Wrzodek, Clemens ; Hahne, Florian ; Zollinger, Tulipan ; Brasa, Sarah ; Kalteis, Magdalena ; Marcellin, Magali ; Giudicelli, Fanny ; Braeuning, Albert ; Morawiec, Laurent ; Zamurovic, Natasa ; Längle, Ulrich ; Scheer, Nico ; Schübeler, Dirk ; Goodman, Jay ; Chibout, Salah-Dine ; Marlowe, Jennifer ; Theil, Dietlinde ; Heard, David J. ; Grenet, Olivier ; Zell, Andreas ; Templin, Markus F. ; Meehan, Richard R. ; Wolf, Roland C. ; Elcombe, Clifford R. ; Schwarz, Michael ; Moulin, Pierre ; Terranova, Rémi ; Moggs, Jonathan G.
The molecular events during nongenotoxic carcinogenesis and their temporal order are poorly understood but thought to include long-lasting perturbations of gene expression. Here, we have investigated the temporal sequence of molecular and pathological perturbations at early stages of phenobarbital (PB) mediated liver tumor promotion in vivo. Molecular profiling (mRNA, microRNA [miRNA], DNA methylation, and proteins) of mouse liver during 13 weeks of PB treatment revealed progressive increases in hepatic expression of long noncoding RNAs and miRNAs originating from the Dlk1-Dio3 imprinted gene cluster, a locus that has recently been associated with stem cell pluripotency in mice and various neoplasms in humans. PB induction of the Dlk1-Dio3 cluster noncoding RNA (ncRNA) Meg3 was localized to glutamine synthetase-positive hypertrophic perivenous hepatocytes, sug- gesting a role for β-catenin signaling in the dysregulation of Dlk1-Dio3 ncRNAs. The carcinogenic relevance of Dlk1-Dio3 locus ncRNA induction was further supported by in vivo genetic dependence on constitutive androstane receptor and β-catenin pathways. Our data identify Dlk1-Dio3 ncRNAs as novel candidate early biomarkers for mouse liver tumor promotion and provide new opportunities for assessing the carcinogenic potential of novel compounds.